文献类型：期刊文献

英文题名：The purification and identification of immunoregulatory peptides from oyster (Crassostrea hongkongensis) enzymatic hydrolysate

作者：Li, Wan[1,5];Xu, Cheng[1,5];Zhang, Chaohua[1,2,3,4,5];Cao, Wenhong[1,2,3,4,5];Qin, Xiaoming[1,2,3,4,5];Gao, Jialong[1,2,3,4,5];Zheng, Huina[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, A321 Food Sci Bldg, Zhanjiang 524088, Peoples R China;[2]Natl Res & Dev Branch, Ctr Shellsh Proc, Zhanjiang 524088, Peoples R China;[3]Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang 524088, Peoples R China;[4]Guangdong Higher Educ Inst, Key Lab Adv Proc Aquat Prod, Zhanjiang 524088, Peoples R China;[5]Guangdong Ocean Univ, Shenzhen Inst, Shenzhen 518108, Peoples R China

年份：2019

卷号：9

期号：56

起止页码：32854

外文期刊名：RSC ADVANCES

收录：SCI-EXPANDED(收录号：WOS:000495961000056)、、WOS

基金：This work was supported by the Applied Science and Technology Research and Development Project of Guangdong Province (2016B020235002), the Modern Agro-industry Technology Research System of China (CARS-49), the Guangdong Higher Education Institution Innovative Team of High Value Processing and Utilization of Aquatic Products (GDOU2016030503) and the Industrial Development Special Fund Project of Dapeng New District, Shenzhen, China (KY20180111). We gratefully acknowledge and are indebted to the anonymous referees for their comments and constructive suggestions provided for improving the manuscript.

语种：英文

外文关键词：Antigen-antibody reactions - Macrophages - Molluscs - Purification - Shellfish

外文摘要：Oysters, favored as a delicious seafood by people worldwide, contain various nutritional components, especially proteins. In this work, the immunoregulatory peptides were isolated and purified from oyster enzymatic hydrolysate by utilizing anion exchange chromatography and RP-HPLC, and were identified via LC-MS/MS. A proliferation assay, a phagocytosis assay, and TNF-alpha production, NO production and IL-6 production assays were used to examine the immunoregulatory effects of the two identified peptides. The results indicate that the peptides, DNSIAMESMK (P1) and LLQLGSGR (P2), increased the proliferation rate of macrophages, TNF-alpha and NO production, IL-6 production, and the phagocytosis ability, but to different degrees; P2, with more hydrophobic amino acids, was more potent than P1. This suggested that these peptides might be potential candidates for developing immunoregulatory functional foods and nutraceuticals.