文献类型：期刊文献

英文题名：Surveillance and cross-species transmission assessment of H3NX avian influenza viruses isolated in Guangdong province, China from 2023 to 2025

作者：Liu, Jie[1];Xiang, Hai yang[2];Liu, Jing[1];Li, Yuan Guo[2];Liang, Xiaodong[3];Yang, Qiong[4];Xie, Jiali[1];Gao, Yuwei[2];Ge, Ye[1]

机构：[1]Guangdong Ocean Univ, Coll Coastal Agr Sci, Zhanjiang, Peoples R China;[2]Chinese Acad Agr Sci, Changchun Vet Res Inst, State Key Lab Pathogen & Biosecur, Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun 130122, Peoples R China;[3]Forestry Adm Guangdong Prov, Guangzhou, Peoples R China;[4]Guangdong Neilingding Futian Natl Nat Researve, Shenzhen, Peoples R China

年份：2026

卷号：105

期号：7

外文期刊名：POULTRY SCIENCE

收录：SCI-EXPANDED(收录号：WOS:001737498600001)、、WOS

基金：The authors thank the authors and laboratories that submitted the AIV sequences to the GISAID and NCBI databases. This work was supported by Guangdong Natural Science Foundation, General Program (grant number: 2024A1515010056) and the Natural Resource Affairs (Ecological Forestry Construction) Project of the Guangdong Forestry Department (grant number: K25032 and K24181) and this work was also supported by the Science and Technology Development Fund of Macau SAR under Grant FDCT 0002/2024/RDP. We thank the Guangdong Neilingding Futian National Nature Researve, Guangdong Zhanjiang Mangrove National Nature Reserve and Leizhou Forestry administration for facilitating our fieldwork.

语种：英文

外文关键词：Avian influenza virus; H3NX; Phylogenetic analysis; Receptor binding; Public health

外文摘要：Continued influenza surveillance remains important, especially given that the emergence of novel subtypes or reassorted influenza viruses with pandemic potential continues to be a worldwide threat. In particular, virus circulating in birds can facilitate interspecies transmission to humans. In this study, we conducted systematic surveillance of H3 subtype avian influenza virus (AIVs) in domestic poultry and wild birds throughout Guangdong Province from 2023 to 2025. A total of 21 strains of H3 subtype AIVs were isolated, and phylogenetic analyses and risk assessment of their internal gene segments revealed genetic evidence of reassortment events, indicating a close genetic relationship with highly pathogenic avian influenza viruses (HPAIVs). ZJ1722, ZJ1542 and SZ837 showed dual-receptor binding ability and robust replication in mammalian cells, which coincided with amino acid mutations in the HA protein associated with human receptor binding. Although the H3NX viruses isolated in this study failed to cause lethality in mice, they efficiently replicated in the nasal turbinate and lungs of mice without prior adaptation. This study highlights the paramount importance of sustained, subtype-specific surveillance targeting H3NX avian influenza viruses coupled with timely risk characterization and assessment. Proactive containment of H3NX avian influenza virus (AIV) transmission has vital implications for safeguarding the sustainability of the poultry industry and protecting global human public health, given the inherent zoonotic potential and evolutionary plasticity of this H3 subtype, which could drive future spillover events.