文献类型：期刊文献

英文题名：Microfluidics for the rapid detection of Staphylococcus aureus using antibody-coated microspheres

作者：Song, Bo[1];Wang, Junsheng[2];Yan, Zhijun[2];Liu, Zhijian[2];Pan, Xinxiang[3];Zhang, Yingbo[1];Zhang, Xiaojie[1]

机构：[1]Qiqihar Med Univ, Med Technol Coll, Dept Clin Pathogen Biol, Qiqihar, Heilongjiang, Peoples R China;[2]Dalian Maritime Univ, Dept Informat Sci & Technol, Dalian, Liaoning, Peoples R China;[3]Guangdong Ocean Univ, Maritime Coll, Zhanjiang, Peoples R China

年份：2020

卷号：11

期号：1

起止页码：1137

外文期刊名：BIOENGINEERED

收录：SCI-EXPANDED(收录号：WOS:000579158500001)、、WOS

基金：Scientific research project of Heilongjiang health and Family Planning Commission 2017-281. Xiaojie Zhang

语种：英文

外文关键词：Microfluidic chip; detection of Staphylococcus aureus; microspheres

外文摘要：Staphylococcus aureusis a common foodborne pathogenic microorganism which can cause food poisoning and it is pathogenic to both humans and animals. Therefore, rapid detection ofS. aureusinfection is of great significance. In this study, a microfluidic platform was introduced to detectS. aureusby fluorescence labeling method and a self-made microfluidic chip, which has immune spheres were used to study the effect of capturingS. aureus. Through this experiment, we found that the platform can be used for microbial culture, andS. aureusantibody coated on the diameter of 50 similar to 90 mu m microspheres for detection. On the premise of optimizing the sample flow rate and detection time, the bacterial detection was quantitatively monitored. Results showed that our platform can detectS. aureusat injection rate of 5 mu L center dot min(-1)reacted for 4 min and the detection limit of bacteria is 1.5 x 10(1)CFU/mu L. However, the detection time of traditional method is 24 hs to 72 hs, and the operation is complex and cumbersome. These findings indicated that the microfluidic chip in this study is portable, sensitive, and accurate, laying a good foundation for further research on the application of rapid bacterial detection platform.