文献类型：期刊文献

英文题名：LC-MS and Transcriptome Analysis of Lipopeptide Biosynthesis by Bacillus velezensis CMT-6 Responding to Dissolved Oxygen

作者：Deng, Qi[1,2];Lin, Haisheng[1,2];Hua, Meifang[1,2];Sun, Lijun[1,2];Pu, Yuehua[1];Liao, Jianmeng[3];Fang, Zhijia[1,2];Zhong, Saiyi[1,2];Gooneratne, Ravi[4]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Coll Food Sci & Technol,Guangdong Prov Engn Techn, Guangdong Prov Engn Lab Marine Biol Prod,Key Lab, Zhanjiang 524088, Peoples R China;[2]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China;[3]Zhanjiang Inst Food & Drug Control, Zhanjiang 524022, Peoples R China;[4]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, POB 85084, Lincoln 7647, New Zealand

年份：2022

卷号：27

期号：20

外文期刊名：MOLECULES

收录：SCI-EXPANDED(收录号：WOS:000873272800001)、、Scopus(收录号：2-s2.0-85140874277)、WOS

基金：This research was funded by the Characteristic Innovation Project of University Colleges in Guangdong Province (No. 2020KQNCX025), Zhanjiang Competitive Allocation Project of Science and Technology Development Special Fund (No. 200915134541577) and the Innovative Team Program of High Education of Guangdong Province (2021KCXTD021).

语种：英文

外文关键词：surfactin and iturin; dissolved oxygen; transcriptomic; LC-MS

外文摘要：Dissolved oxygen (DO) is an key factor for lipopeptide fermentation. To better understand the link between oxygen supply and lipopeptide productivity in Bacillus velezensis CMT-6, the mechanism of DO on the synthesis of antimicrobial lipopeptides by Bacillus velezensis CMT-6 was examined. The production of surfactin and iturin of CMT-6 was detected by liquid chromatography-mass spectrometer (LC-MS) under different DO conditions and transcriptome analysis was performed. At 100 and 200 rpm, the lipopeptides productions were 2753.62 mg/L and 3452.90 mg/L, respectively. There was no significant change in the yield of iturin but that of surfactin increased by 64.14%. Transcriptome analysis revealed that the enriched differential genes were concentrated in the GO term of oxidation-reduction process. The marked enrichment of the lipopeptides synthesis pathway, including microbial metabolism in diverse environments and carbon metabolism in the two-component system, were observed. More importantly, the expression levels of the four surfactin synthetase genes increased at higher DO, however, the iturin synthetase gene expression did not. Furthermore, modular surfactin synthetase was overexpressed (between 9- and 49-fold) at 200 rpm but not at 100 rpm, which is suggestive of efficient surfactin assembly resulting in surfactin overproduction. This study provides a theoretical basis for constructing engineering strains with high lipopeptide production to adapt to different DO.