文献类型：期刊文献

英文题名：Reproductive effects of synthetic progestin norgestrel in zebrafish (Danio rerio)

作者：Liang, Yan-Qiu[1]; Huang, Guo-Yong[2]; Lin, Zhong[1]; Li, Jin[1]; Yang, Jie-Wen[1]; Zhong, Lai-Yuan[1]; Ying, Guang-Guo[2,3]

机构：[1] Faculty of Chemistry and Environmental Science, Guangdong Ocean University, Zhanjiang, 524088, China; [2] State Key Laboratory of Organic Geochemistry, CAS Research Centre of PRD Environmental Pollution and Control, Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou, 510640, China; [3] The Environmental Research Institute, MOE Key Laboratory of Environmental Theoretical Chemistry, South China Normal University, Guangzhou, 510006, China

年份：2018

卷号：190

起止页码：17

外文期刊名：Chemosphere

收录：EI(收录号：20174004240433)、Scopus(收录号：2-s2.0-85030328934)

语种：英文

外文关键词：Transcription

外文摘要：The aim of this study was to assess the adverse effects of synthetic progestin norgestrel (NGT) on the reproduction of zebrafish by measuring the egg production, histology and transcriptional expression profiles along the hypothalamic-pituitary-gonadal (HPG) axis in adult zebrafish. After a pre-exposure period of 7 days, adult zebrafish were exposed to 6, 29 and 69 ng L?1 NGT for 21 days. The results showed that exposure to 69 ng L?1 NGT led to a significant up-regulation of follicle stimulating hormone, beta polypeptide (fshb), luteinizing hormone, beta polypeptide (lhb), progesterone receptor (pgr), estrogen receptor 1 (esr1) and androgen receptor (ar) genes in the brains, as well as significant up-regulation of hydroxysteroid 20-beta dehydrogenase (hsd20b) and hydroxysteroid 11-beta dehydrogenase 2 (hsd11b2) genes and down-regulation of 11-beta-hydroxylase (cyp11b) gene in the ovaries of females. In the testes of males, an overall down-regulation of steroidogenic acute regulatory protein (star), cytochrome P450-mediated side-chain cleavage enzyme (cyp11a1), cyp11b, hsd20b, hydroxysteroid 17-beta dehydrogenase type 3 (hsd17b3), hsd11b2 and ar genes were observed following exposure to different treatments of NGT. These transcriptional alterations imply that NGT could exhibit the potent progestogenic and androgenic activities in zebrafish. Egg production as well as histology in the ovaries and testes was not affected by NGT. Taken together, the overall results demonstrated that NGT could significantly affect transcriptional expression levels of genes related to HPG axis in zebrafish, and whether that change translates to additional physiological effects is needed further research. ? 2017