文献类型：期刊文献

英文题名：Effect of black soldier fly (Hermetia illucens) larvae meal on lipid and glucose metabolism of Pacific white shrimp Litopenaeus vannamei

作者：Chen, Yongkang[1];Chi, Shuyan[1,2,3];Zhang, Shuang[1,2,3];Dong, Xiaohui[1,2,3];Yang, Qihui[1,2,3];Liu, Hongyu[1,2,3];Tan, Beiping[1,2,3];Xie, Shiwei[1,2,3]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Nutr & Feed, Zhanjiang 524088, Peoples R China;[2]Aquat Anim Precis Nutr & High Efficiency Feed Eng, Zhanjiang 524088, Peoples R China;[3]Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Tech, Zhanjiang 524088, Peoples R China

年份：2022

卷号：128

期号：9

起止页码：1674

外文期刊名：BRITISH JOURNAL OF NUTRITION

收录：SCI-EXPANDED(收录号：WOS:000742221400001)、、Scopus(收录号：2-s2.0-85120644174)、WOS

基金：The authors thank all those who helped in shrimp culture, sampling and testing. This study was financially supported by the fund of The National Key R&D Program of China (2019YFD0900200), National Natural Science Foundation of China (32002402) and Guangdong Basic and Applied Basic Research Foundation (2019A1515011970, 2021A1515010428). Y. C designed the study, carried out the study, analysed the data, and drafted and revised the article. S. C assisted in the correction and developed the questions. S. Z. designed the experiment. X. D. designed the experiment and assisted in the correction. Q. Y. designed the experiment, assisted in the correction and developed the questions. H. L. designed the experiment. B. T. approved the final version to be published and agreed to be accountable for all aspects of the work. S. X. designed the experiment, revised the manuscript critically for important intellectual content and approved the final version to be published. All authors read and approved the final version of the manuscript. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

语种：英文

外文关键词：Litopenaeus vannamei; Black soldier fly; Fishmeal replacement; Lipid metabolism; Glucose metabolism

外文摘要：The present study investigated the effect of black soldier fly (Hermetia illucens) larvae meal (BSF) on haemolymph biochemical indicators, muscle metabolites as well as the lipid and glucose metabolism of Pacific white shrimp Litopenaeus vannamei. Four diets were formulated in which the control diet contained 25 % of fishmeal (FM) and 10 % (BSF10), 20 % (BSF20), and 30 % (BSF30) of FM protein were replaced with BSF. Four hundred and eighty shrimp (0 center dot 88 +/- 0 center dot 00 g) were distributed to four groups of three replicates and fed for 7 weeks. Results showed that growth performance of shrimp fed BSF30 significantly decreased compared with those fed FM, but there was no significant difference in survival among groups. The whole shrimp crude lipid content, haemolymph TAG and total cholesterol were decreased with the increasing BSF inclusion. The results of metabolomics showed that the metabolite patterns of shrimp fed different diets were altered, with significant changes in metabolites related to lipid metabolism, glucose metabolism as well as TCA cycle. The mRNA expressions of hk, pfk, pk, pepck, ampk, mcd, cpt-1 and scd1 in hepatopancreas were downregulated in shrimp fed BSF30, but mRNA expression of acc1 was upregulated. Unlike BSF30, the mRNA expressions of fas, cpt-1, fbp and 6pgd in hepatopancreas were upregulated in shrimp fed BSF20. This study indicates that BSF20 diet promoted lipid synthesis and lipolysis, while BSF30 diet weakened beta-oxidation and glycolysis as well as affected the unsaturated fatty acids synthesis, which may affect the growth performance and body composition of shrimp.