文献类型：期刊文献

中文题名：基于脯氨酸合成积累的甘蔗分蘖期耐旱生理效应分析

英文题名：Analysis of Drought to Lerance Physiological Effect Based on Proline Biosynthesis and Accumulation at the Tillering Stage in Sugarcane

作者：杨善[1];杨杰文[1];叶昌辉[1];莫俊杰[1];周鸿凯[1]

机构：[1]广东海洋大学农学院

年份：2015

卷号：35

期号：6

起止页码：87

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：国家自然科学基金(41073059);广东省科技计划项目(2013B020301005;2013B020301008);广东省良种培育和引进专项(201201148)

语种：中文

中文关键词：甘蔗;脯氨酸;P5CS;δ-OAT;干旱胁迫

外文关键词：sugarcane; proline; P5CS; δ-OAT; drought stress

中文摘要：为探究干旱胁迫下分蘖期甘蔗植株内游离脯氨酸积累及其合成关键酶的响应机制,以甘蔗品种ROC22为材料进行桶栽干旱胁迫实验,测定生长于7种不同土壤水分下的甘蔗叶片中丙二醛含量、电导率、游离脯氨酸含量以及δ-OAT、P5CS、SOD的活性。结果表明,随着干旱胁迫强度增大,植株体内不同程度地激活了P5CS、δ-OAT、SOD的活性,合成积累了大量的游离脯氨酸,以抵抗丙二醛等氧化物的积累所造成对细胞膜透性的破坏。但在沙土的土壤水分为2.64%(相对持水量21.2%)时,达到了ROC22的萎蔫系数。相关统计分析结果,6个甘蔗生理指标间的简单正相关均达到了显著水平。偏相关分析结果,细胞膜透性分别与P5CS活性、脯氨酸含量为负的偏相关且达到了显著水平,丙二醛、δ-OAT、SOD分别与脯氨酸含量的偏相关系数均到达了极显著水平。此外,P5CS与SOD存在共线性,多元相关性分析表明P5CS对游离脯氨酸积累的作用显著地大于δ-OAT,在干旱胁迫下,甘蔗脯氨酸的两个合成途径表现为以Glu→Pro途径主,Orn→Pro途径为辅。

外文摘要：To explore the responses of free proline accumulation and its synthesis key enzymes at the tillering stage in sugarcane under drought stress,sugarcane variety ROC22 as materials was cultivated in bucket for drought stress test. The malondialdehyde content, conductivity, free proline content and the activity of δ-OAT, P5CS and SOD were measured under the seven soil moisture levels in sugarcane leaves.The results showed that when drought stress strength increased, the activity of P5CS, δ-OAT and SOD were activated in different degree, synthesized and accumulated a large amount of free proline, to resist the damage caused by accumulation of MDA and other oxides for the cell membrane permeability. But when soil moisture was 2.64%（relative water capacity was 21.2%） in sandy soil, the wilting coefficient reached up to ROC22. Statistical analysis of the results showed that the simple correlation between 6 physiological indexes of sugarcane had reached a significant level. Further partial correlation analysis showed that the cell membrane permeability and P5CS, proline content were negative partial correlation and reached a significant level. MDA, δ-OAT, SOD and proline content of partial correlation coefficients had reached a significant level. Meanwhile, SOD and P5CS were collinear and the multiple correlation analysis showed that the effects excerted on proline accumulation by P5CS was significantly greater than that by δ-OAT, and it appeared that the glutamic acid pathway（Glu→Pro）was the main pathway, and ornithine pathway（Orn→Pro）supplemented under drought stress in sugarcane.