文献类型：期刊文献

英文题名：Proteomic profiling of serum extracellular vesicles identifies diagnostic markers for echinococcosis

作者：Guo, Xiaola[1];Wang, Shuai[1];Zhang, Junmei[1];Li, Rui[1];Zhang, Yong'e[1];Wang, Zhengrong[2];Kong, Qingming[3];Cho, William[4];Ju, Xianghong[5];Shen, Yujuan[6];Zhang, Lingqiang[7];Fan, Haining[7];Cao, Jianping[6];Zheng, Yadong[8,9]

机构：[1]Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Vet Parasitol Gansu Prov, Lanzhou, Peoples R China;[2]Xinjiang Acad Agr & Reclamat Sci, State Key Lab Sheep Genet Improvement & Hlth Prod, Shihezi, Peoples R China;[3]Hangzhou Med Coll, Inst Parasit Dis, Sch Biol Engn, Hangzhou, Peoples R China;[4]Queen Elizabeth Hosp, Dept Clin Oncol, Hong Kong, Peoples R China;[5]Guangdong Ocean Univ, Coll Agr, Dept Vet Med, Zhanjiang, Peoples R China;[6]Chinese Ctr Dis Control & Prevent, Natl Hlth Commiss Peoples Republ China, Natl Inst Parasit Dis, Key Lab Parasite & Vector Biol,Chinese Ctr Trop Di, Shanghai, Peoples R China;[7]Qinghai Univ, Dept Hepatopancreatobiliary Surg, Qinghai Prov Key Lab Hydatid Dis Res, Affiliated Hosp, Xining, Peoples R China;[8]Zhejiang A&F Univ, Key Lab Appl Technol Green Ecohlth Anim Husb Zheji, Zhejiang Prov Engn Lab Anim Hlth Inspection & Inte, China Australia Joint Lab Anim Hlth Big Data Analy, Hangzhou, Peoples R China;[9]Zhejiang A&F Univ, Coll Vet Med, Hangzhou, Peoples R China

年份：2022

卷号：16

期号：10

外文期刊名：PLOS NEGLECTED TROPICAL DISEASES

收录：SCI-EXPANDED(收录号：WOS:000934966600001)、、Scopus(收录号：2-s2.0-85140414837)、WOS

基金：The study was financially supported by grants from the State Key Laboratory of Sheep Genetic Improvement and Healthy Production (Grant No. MYSKLKF202003 to XG), the Agricultural Science and Technology Innovation Program (Grant No. CAAS-ASTIP-2016-LVRI-03 to SW), and Scientific Research and Development Talent Fund of Zhejiang Agriculture and Forestry University (Grant No. 2021LFR038 to YZ). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文摘要：Echinococcosis is a parasitic disease caused by the metacestodes of Echinococcus spp. The disease has a long latent period and is largely underdiagnosed, partially because of the lack of effective early diagnostic approaches. Using liquid chromatography-mass spectrometry, we profiled the serum-derived extracellular vesicles (EVs) of E. multilocularis-infected mice and identified three parasite-origin proteins, thioredoxin peroxidase 1 (TPx-1), transitional endoplasmic reticulum ATPase (TER ATPase), and 14-3-3, being continuously released by the parasites into the sera during the infection via EVs. Using ELISA, both TPx-1 and TER ATPase were shown to have a good performance in diagnosis of experimental murine echinococcosis as early as 10 days post infection and of human echinococcosis compared with that of control. Moreover, TER ATPase and TPx-1 were further demonstrated to be suitable for evaluation of the prognosis of patients with treatment. The present study discovers the potential of TER ATPase and TPx-1 as promising diagnostic candidates for echinococcosis. Author summaryEchinococcosis, also known as hydatid disease, is one of the neglected zoonotic diseases. Echinococcus multilocularis and Echinococcus granulosus sensu latu are the causative agents responsible for alveolar echinococcosis and cystic echinococcosis, respectively. Alveolar echinococcosis is mainly endemic in the areas of the northern hemisphere, whereas cystic echinococcosis has a worldwide distribution. The disease has a long latent period of up to 5 to 10 years and is largely underdiagnosed, partially due to the lack of effective early diagnostic approaches. In recent years, an emerging role of EVs in intercellular communication and diagnosis has been recognized. Here, we employed a proteomic approach to identify parasite-derived proteins in the serum EVs from E. multilocularis-infected mice and assessed their diagnostic values. This study signify the role of EVs for the identification of diagnostic candidates by the discovery of two identified proteins, TER ATPas and TPX-1. First results indicate their diagnostic and prognostic values in experimental murine and human echinococcosis.