文献类型：期刊文献

中文题名：盐酸小檗碱含药血清与血浆抗鼻咽癌CNE-1细胞作用的比较分析

英文题名：Anti-tumor effect of berberine hydrochloride in the serum and plasma on nasopharyngeal carcinoma CNE-1 cells:A comparative study

作者：李彩虹[1];彭罡[1];李锦云[2];王效军[3];徐德峰[4];周克元[1]

机构：[1]广东医学院基础医学院,东莞医学博士研究生523808;[2]海南通用三洋药业有限公司,海口570132;[3]广东医学院公共卫生学院,东莞523808;[4]广东海洋大学食品科技学院,湛江524088

年份：2013

卷号：26

期号：7

起止页码：676

中文期刊名：医学研究生学报

外文期刊名：Journal of Medical Postgraduates

收录：CSTPCD、、北大核心2011、北大核心

基金：国家自然科学基金(81272434);广东医学院科研基金(XK1306);广东省中医药局科研课题(2010410)

语种：中文

中文关键词：盐酸小檗碱;血清药理学;血浆药理学;生长抑制;诱导凋亡

外文关键词：Berberine hydrochloride ; Seropharmacology ; Plasmopharmacology; Growth inhibition ; Apoptosis induction

中文摘要：目的盐酸小檗碱体外抗肿瘤等作用活性显著,目前针对其从体外过渡到体内的研究偶见报道,文中研究通过半体内研究方法,比较盐酸小檗碱含药血清、血浆对人鼻咽癌CNE-1细胞的生长抑制及诱导凋亡作用。方法新西兰白兔灌胃给药,分离血清、血浆后,56℃灭活30 min,制成含药血清、血浆,采用反向高效液相色谱法(rerersed phase high performancel iquid chromatography,RP-HPLC)分析盐酸小檗碱含量,采用MTT法比较不同作用时间和剂量对鼻咽癌CNE-1细胞增殖的抑制作用,用Annexin v-FITC流式细胞术观察诱导细胞凋亡情况。结果盐酸小檗碱含药血清、血浆均能够抑制CNE-1细胞增殖,含药血浆的抑制作用强于含药血清,且含药血浆能显著诱导CNE-1细胞凋亡。结论凡是使用血清与血浆均可的实验,应当优先选用含药血浆。

外文摘要：Objective Berberine hydrochloride （BH） has an obvious anti-tumor activity in vitro, and studies on its effect are reportedly from in vitro to in vivo. This article aims to compare the inhibiting and apoptosis-inducing effects of BH in the serum and plasma on nasopharyngeal carcinoma CNE-I cells using semi-in vivo research methods. Methods New Zealand rabbits were given BH by gavage, and then the serum and plasma were separated from the animals and inactivated in 56~C water for 30 minutes. The con- tents of BH in the serum （SBH） and plasma （PBH） were determined by RP-HPLC, the effects SBH and PBH on the proliferation of the CNE-1 cells evaluated by MTr assay, and the apoptosis of the cells detected using Annexin V-FITC flow cytometry. Results Both SBH and PBH suppressed the growth of the CNE-1 cell line, and PBH exhibited an even better inhibitory effect than SBH. Be- sides, PBH induced a significant apoptosis of the CNE-1 cells. Conclusion SBH and PBH can inhibit the proliferation and induce the apoptosis of CNE-1 cells, and PBH has even better effects than SBH.