文献类型：期刊文献

英文题名：Taurine Promotes Milk Synthesis via the GPR87-PI3K-SETD1A Signaling in BMECs

作者：Yu, Mengmeng[1];Wang, Yang[2];Wang, Zhe[2];Liu, Yanxu[2];Yu, Yang[2];Gao, Xuejun[1]

机构：[1]Guangdong Ocean Univ, Agr Coll, Zhanjiang 524088, Peoples R China;[2]Northeast Agr Univ, Key Lab Dairy Sci, Educ Minist, Harbin 150030, Heilongjiang, Peoples R China

年份：2019

卷号：67

期号：7

起止页码：1927

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000459641900015)、、EI(收录号：20190706511392)、Scopus(收录号：2-s2.0-85061497983)、WOS

基金：This research was jointly supported by grants from the National Natural Science Foundation of China (Grants 31671473 and 31472162).

语种：英文

外文关键词：mammary epithelial cells; GPR87; milk synthesis; PI3K; SETD1A; taurine

外文摘要：Taurine, a beta-aminosulfonic acid, exerts many cellular physiological functions. It is still unknown whether taurine can regulate milk synthesis in the mammary gland. Therefore, in this study we investigated the effects and mechanism of taurine on milk synthesis in mammary epithelial cells (MECs). Bovine MECs (BMECs) cultured in FBS-free OPTI-MEMImedium were treated with taurine (0, 0.08, 0.16, 0.24, 0.32, and 0.4 mM). Taurine treatment led to increased milk protein and fat synthesis, mTOR phosphorylation, and SREBP-1c protein expression, in a dose-dependent manner, with an apparent maximum at 0.24 mM. Gene function study approaches revealed that the GPR87-PI3K-SETD1A signaling was required for taurine to increase the mTOR and SREBP-1c mRNA levels. Taurine stimulated GPR87 expression and cell membrane localization in a dose dependent manner, suggesting a sensing mechanism of GPR87 to extracellular taurine. Collectively, these data demonstrate that taurine promotes milk synthesis via the GPR87-PI3K-SETD1A signaling.