文献类型：期刊文献

英文题名：Distinct functions of neuromedin u and neuromedin s in orange-spotted grouper

作者：Li, Shuisheng[1,2];Xiao, Ling[1,2];Liu, Qiongyu[1,2];Zheng, Binbin[1,2];Chen, Huapu[3];Liu, Xiaochun[1,2];Zhang, Yong[1,2];Lin, Haoran[1,2]

机构：[1]Sun Yat Sen Univ, State Key Lab Biocontrol, Inst Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[3]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China

年份：2015

卷号：55

期号：2

起止页码：95

外文期刊名：JOURNAL OF MOLECULAR ENDOCRINOLOGY

收录：SCI-EXPANDED(收录号：WOS:000365033300005)、、WOS

基金：This study was supported by the National '863' Program of China (2012AA10A407), the National Natural Science Foundation of China (31261160493), the Special Fund for Fisheries-Scientific Research of Guangdong Province (A201400A01, A201501A03 and A201501A09), the Project of Science and Technology New Star in Zhujiang city (2013J2200093), the Fundamental Research Funds for the Central Universities (151gzs102 and 151gzs121), and the Hainan Province Marine Science and technology foundation (XH201401).

语种：英文

外文关键词：nmu; nms; structural diversity; feeding regulation; pituitary function

外文摘要：Neuromedin U (NMU) and neuromedin S (NMS) play inhibitory roles in the regulation of food intake and energy homeostasis in mammals. However, their functions are not clearly established in teleost fish. In the present study, nmu and nms homologs were identified in several fish species. Subsequently, their cDNA sequences were cloned from the orange-spotted grouper (Epinephelus coioides). Sequence analysis showed that the orange-spotted grouper Nmu proprotein contains a 21-amino acidmature Nmu peptide(Nmu-21). The Nms proprotein lost the typical mature Nms peptide, but it retains a putative 34-amino acid peptide (Nmsrp). In situ hybridization revealed that nmu- and nms-expressing cells are mainly localized in the hypothalamic regions associated with appetite regulation. Food deprivation decreased the hypothalamic nmu mRNA levels but induced an increase of nms mRNA levels. Periprandial expression analysis showed that hypothalamic expression of nmu increased significantly at 3 h post-feeding, while nms expression was elevated at the normal feeding time. I.p. injection of synthetic Nmu-21 peptide suppressed the hypothalamic neuropeptide y (npy) expression, while Nmsrp administration significantly increased the expression of npy and orexin in orange-spotted grouper. Furthermore, the mRNA levels of LH beta subunit (lh beta) and gh in the pituitary were significantly down-regulated after Nmu-21 peptide administration, while Nmsrp was able to significantly stimulate the expression of FSH beta subunit (fsh beta), prolactin (prl), and somatolaction (sl). Our results indicate that nmu and nms possess distinct neuroendocrine functions and pituitary functions in the orange spotted grouper.