文献类型：期刊文献

英文题名：Effects of dietary chitosan and Bacillus subtilis on the growth performance, non-specific immunity and disease resistance of cobia, Rachycentron canadum

作者：Geng, Xu[1];Dong, Xiao-Hui[1];Tan, Bei-Ping[1];Yang, Qi-Hui[1];Chi, Shu-Yan[1];Liu, Hong-Yu[1];Liu, Xian-Qin[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Lab Aquat Anim Nutr & Feed, Zhanjiang 524025, Guangdong, Peoples R China

年份：2011

卷号：31

期号：3

起止页码：400

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000294150800005)、、Scopus(收录号：2-s2.0-79960639202)、WOS

基金：This study was financially supported by Department of Education of Guangdong Province and Guangdong Ocean University. The authors wish to extend their sincerest appreciation to Jia-Li Han Liu-Jian He, Jing-Qiang He, Bao-Shu Xu, Yi-Hong Chen and Wen-Jiao Meng for their assistance in the experiment.

语种：英文

外文关键词：Rachycentron canadum; Chitosan; Bacillus subtilis; Immunity; Growth; Vibrio harveyi

外文摘要：The present study was performed to investigate the effects of various levels of dietary Bacillus sub tills and chitosan on the growth performance, non-specific immunity and protection against Vibrio harveyi infection in cobia, Rachycentron canadum. Fish were fed with the control diet and six different experimental diets containing three graded levels of B. subtilis at 2 x 10(10) CFU g(-1) (0.0, 1.0, 2.0 g kg(-1) diet) for each of two levels of chitosan (3.0 and 6.0 g kg(-1) diet). The results of 8 weeks feeding trial showed that the survival rate ranged from 81.3% to 84.0% with no significant difference (P > 0.05). The SGR (%) in the fish fed with dietary treatments was significantly higher than that of the control fish except diet 6 group with 2.0 g kg(-1) B. subtilis and 3.0 g kg(-1) chitosan. The serum lysozyme activities were significantly higher in 6.0 g kg(-1) chitosan groups and no significant differences were observed among B. subtilis levels. The serum ACP activities were significantly higher in 3.0 g kg(-1) chitosan groups at 0.0 and 1.0 g kg(-1) B. subtilis levels; at low chitosan level, the cobia fed diets with 1.0 g kg(-1) B. subtilis had significantly higher serum ACP activity, but at high chitosan level, the cobia fed diets with 2.0 g kg(-1) B. subtilis had significantly higher serum ACP activity. The phagocytosis and respiratory burst activity in the fish fed with dietary treatments was significantly higher than that of the control fish except diet 3 group with 6.0 g kg(-1) chitosan. Moreover, fish fed the diet containing 2.0 g kg(-1), B. subtilis and 6.0 g kg(-1) chitosan had significantly higher post-challenge survival on the 7th day following V. harveyi infection and post-challenge survival showed clearly the synergistic effect of chitosan and B. subtilis. Based on these results, the combination of 1.0 g kg(-1) B. subtilis and 6.0 g kg(-1) chitosan is optimal for the growth, innate immunity and disease resistance of cobia with an 8-week oral administration. (C) 2011 Elsevier Ltd. All rights reserved.