文献类型：期刊文献

英文题名：Molecular cloning of the insulin-like growth factor 3 and difference in the expression of igf genes in orange-spotted grouper (Epinephelus coioides)

作者：Yang, Huirong[1,2,3];Chen, Huapu[4];Zhao, Huihong[3];Liu, Li[3];Xie, Zhenzhen[1,2];Xiao, Ling[1,2];Li, Shuisheng[1,2];Zhang, Yong[1,2];Lin, Haoran[1,2,5]

机构：[1]Sun Yat Sen Univ, Sch Life Sci, Inst Aquat Econ Anim, State Key Lab Biocontrol, Guangzhou 510275, Guangdong, Peoples R China;[2]Sun Yat Sen Univ, Sch Life Sci, Guangdong Prov Key Lab Aquat Econ Anim, Guangzhou 510275, Guangdong, Peoples R China;[3]South China Agr Univ, Anim Sci Coll, Guangzhou 510642, Guangdong, Peoples R China;[4]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[5]Hainan Univ, Coll Ocean, Haikou 570228, Peoples R China

年份：2015

卷号：186

起止页码：68

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000356106700009)、、WOS

基金：This work is supported by the National 863 Program of China (no. 2012AA10A407); The national Spark Program (Application No. S2015E200004); the Guangdong Provincial Science and Technology Program (no. 2013B020308001, no. 2012B090500008); the Special Fund for Agro-Scientific Research in Public Interest (nos. 200903046, 201303048); the Guangdong Provincial Oceanic and Fishery Administration Fund (nos. A201100D01, A201100D03) and the Hainan Provincial Science and Technology Program (no. XH201401).

语种：英文

外文关键词：igf; Gh; hCG; Orange-spotted grouper; Ovarian development

外文摘要：Insulin-like growth factor (Igf) is the key regulator for development, growth, and reproduction. In most vertebrate species, the Igf family has two forms: Igf1 and Igf2. A novel form of Igf, termed Igf3, was recently discovered in fish. In the present study, we isolated igf3 from the orange-spotted grouper (Epinephelus coioides). The orange-spotted grouper igf3 consisted of a full-length cDNA of 1014 nucleotides with an open reading frame (ORF) of 597 bp, encoding for proteins of 199 amino acid residues in length. Tissue distribution analysis showed that igf1 widely expressed with the highest expression in the pituitary and liver. igf1 was expressed highly in all the tissues except the olfactory bulb, while igf3 showed the highest expression in the ovary, and moderate expression in brain areas. The expression profiles of three igf genes during the ovarian development and growth hormone (Gh) and human chorionic gonadotropin (hCG) treatment were also investigated. Three igf genes exhibited different expression patterns during the ovarian development, and showed different responses to the Gh and hCG treatments, appearing to play distinct roles in ovarian development. The present study provides further evidence for the existence of an intraovarian Igf system in orange-spotted grouper. (C) 2015 Elsevier Inc All rights reserved.