文献类型：期刊文献

英文题名：Combined Transcriptome and Metabolome Analysis of Musa nana Laur. Peel Treated With UV-C Reveals the Involvement of Key Metabolic Pathways

作者：Chen, Ming-zhong[1,2];Zhong, Xu-Mei[2];Lin, Hai-Sheng[1];Qin, Xiao-Ming[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Product Proc & Safet, Zhanjiang, Peoples R China; Yangjiang Polytech, Yangjiang, Peoples R China

年份：2022

卷号：12

外文期刊名：FRONTIERS IN GENETICS

收录：SCI-EXPANDED(收录号：WOS:000753774600001)、、WOS

基金：This research was funded by China Agriculture Research System of MOF and MARA; Characteristic innovative technology pro-jects in Guangdong Province (2019GKTSCX122) and Science and Technology Key Project of Yangjiang Polytechnic (2019kjzd02). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：antioxidant activity; banana storage; flavonoid biosynthesis; hypersensitive response; postharvest treatment; UV-C treatment; phenylpropanoid biosynthesis

外文摘要：An increasing attention is being given to treat fruits with ultraviolet C (UV-C) irradiation to extend shelf-life, senescence, and protection from different diseases during storage. However, the detailed understanding of the pathways and key changes in gene expression and metabolite accumulation related to UV-C treatments are yet to be explored. This study is a first attempt to understand such changes in banana peel irradiated with UV-C. We treated Musa nana Laur. with 0.02 KJ/m(2) UV-C irradiation for 0, 4, 8, 12, 15, and 18 days and studied the physiological and quality indicators. We found that UV-C treatment reduces weight loss and decay rate, while increased the accumulation of total phenols and flavonoids. Similarly, our results demonstrated that UV-C treatment increases the activity of defense and antioxidant system related enzymes. We observed that UV-C treatment for 8 days is beneficial for M. nana peels. The peels of M. nana treated with UV-C for 8 days were then subjected to combined transcriptome and metabolome analysis. In total, there were 425 and 38 differentially expressed genes and accumulated metabolites, respectively. We found that UV-C treatment increased the expression of genes in secondary metabolite biosynthesis related pathways. Concomitant changes in the metabolite accumulation were observed. Key pathways that were responsive to UV-C irradiation include flavonoid biosynthesis, phenylpropanoid bios6ynthesis, plant-pathogen interaction, MAPK signaling (plant), and plant hormone signal transduction pathway. We concluded that UV-C treatment imparts beneficial effects on banana peels by triggering defense responses against disease, inducing expression of flavonoid and alkaloid biosynthesis genes, and activating phytohormone and MAPK signaling pathways.