文献类型：期刊文献

英文题名：Integrated transcriptomic and metabolomic analysis sheds new light on adaptation of Pinctada fucata martensii to short-term hypoxic stress

作者：Chen, Jiayi[1];Qiu, Jinyu[1];Yang, Chuangye[1,3,4,5];Liao, Yongshan[3];He, Maoxiao[2];Mkuye, Robert[1];Li, Junhui[1];Deng, Yuewen[1,3,4,5];Du, Xiaodong[1,3,4,5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Chinese Acad Sci, South China Sea Inst Oceanol, CAS Key Lab Trop Marine Bioresources & Ecol, Guangdong Prov Key Lab Appl Marine Biol, Guangzhou 510301, Peoples R China;[3]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[5]Guangdong Marine Ecol Early Warning & Monitoring L, Zhanjiang 524088, Peoples R China

年份：2023

卷号：187

外文期刊名：MARINE POLLUTION BULLETIN

收录：SCI-EXPANDED(收录号：WOS:000917076100001)、、EI(收录号：20230113330767)、Scopus(收录号：2-s2.0-85145217031)、WOS

基金：This work was supported by the Open Fund of Key Laboratory of Tropical Marine Bio-resources and Ecology of Chinese Academy of Science and Guangdong Provincial Key Laboratory of Applied Marine Biology (Grant No. 2021011002) , National Natural Science Foundation of China (Grant No. 32102817) , and Department of Education of Guangdong Province (Grant No. 2020ZDZX1045 and 2021KCXTD026) , the earmarked fund for CARS-49, and Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (Grant No. 2023KJ146) . We thank TopEdit ( www.topeditsci.com ) for linguistic assistance during the preparation of this manuscript. We are very grateful to Marine Pearl Science and Technology Backyard in Leizhou of Guangdong for providing our experimental site. Metabolomics analysis was assisted by Biotree Biotech Co., Ltd. (Shanghai, China) .

语种：英文

外文关键词：Pinctada fucata martensii; Hypoxic stress; Transcriptomics; Metabolomics

外文摘要：Analyses of the transcriptome and metabolome were conducted to clarify alterations of key genes and metab-olites in pearl oysters following exposure to short-term hypoxic treatment. We totally detected 209 DEGs be-tween the control and hypoxia groups. Enrichment analysis indicated the enrichment of GO terms including "oxidation-reduction process", "ECM organization", "chaperone cofactor-dependent protein refolding", and "ECM-receptor interaction" KEGG pathway by the DEGs. In addition, between the two groups, a total of 28 SDMs were identified, which were implicated in 13 metabolic pathways, such as "phenylalanine metabolism", "D -amino acid metabolism", and "aminoacyl-tRNA biosynthesis". Results suggest that pearl oysters are exposed to oxidative stress and apoptosis under short-term hypoxia. Also, pearl oysters might adapt to short-term hypoxic treatment by increasing antioxidant activity, modulating immune and biomineralization activities, maintaining protein homeostasis, and reorganizing the cytoskeleton. The results of our study help unveil the mechanisms by which pearl oysters respond adaptively to short-term hypoxia.