文献类型：期刊文献

英文题名：Reproductive toxic effects of chronic exposure to bisphenol A and its analogues in marine medaka ( Oryzias melastigma )

作者：Chen, Zuchun[1];Li, Xueyou[1];Gao, Jiahao[1];Liu, Yue[1];Zhang, Ning[1];Guo, Yusong[1];Wang, Zhongduo[1];Dong, Zhongdian[1,2]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Higher Educ Inst, Key Lab Aquaculture South China Sea Aquat Econ An, Zhanjiang 524088, Peoples R China;[2]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China

年份：2024

卷号：271

外文期刊名：AQUATIC TOXICOLOGY

收录：SCI-EXPANDED(收录号：WOS:001232397400001)、、Scopus(收录号：2-s2.0-85190751253)、WOS

基金：This research was funded by the National Natural Science Founda-tion of China (41806195) , Nanhai Scholar Project of Guangdong Ocean University (QNXZ201807, 201903) , Postgraduate Education Innovation Project of Guangdong Ocean University (202154, 202451) , and Un-dergraduate Innovation Team of Guangdong Ocean University (CXTD2023004) .

语种：英文

外文关键词：Endocrine disruptors; Bisphenol; Oryzias melastigma; Reproductive toxicity; Intergenerational effect

外文摘要：As awareness of BPA ' s health risks has increased, many countries and regions have implemented strict controls on its use. Consequently, bisphenol analogues like BPF and BPAF are being increasingly used as substitutes. However, these compounds are also becoming increasingly prevalent in the environment due to production, use and disposal processes. The oceans act as a repository for various pollutants, and recent studies have revealed the extensive presence of bisphenols (BPs, including BPA, BPF, BPAF, etc.) in the marine environment, posing numerous health hazards to marine wildlife. Nevertheless, the reproductive toxicity of these chemicals on marine fish is not comprehensively comprehended yet. Thus, the histological features of the gonads and the gene expression profiles of HPG (Hypothalamic-Pituitary-Gonadal) axis-related genes in marine medaka ( Oryzias melastigma ) were studied after exposure to single and combined BPs for 70 days. The effects of each exposure group on spawning, embryo fertilization, and hatching in marine medaka were also assessed. Furthermore, the impacts of each exposure group on the genes related to methylation in the F2 and F3 generations were consistently investigated. BPs exposure was found to cause follicular atresia, irregular oocytes, and empty follicles in the ovary; but no significant lesions in the testis were observed. The expression of several HPG axis genes, including cyp19b, 17 beta hsd, 3 beta hsd , and fshr , resulted in significant changes compared to the control group. The quantity of eggs laid and fertilization rate decreased in all groups treated with BPs, with the BPAF-treated group showing a notable reduction in the number of eggs laid. Additionally, the hatching rate showed a more significant decline in the BPF-treated group. The analysis of methylated genes in the offspring of bisphenol-treated groups revealed significant changes in the expression of genes including amh, dnmt1, dnmt3ab, mbd2 , and mecp2 , indicating a potential transgenerational impact of bisphenols on phenotype through epigenetic modifications. Overall, the potential detrimental impact of bisphenol on the reproduction of marine medaka emphasizes the need for caution in considering the use of BPAF and BPF as substitutes.