文献类型：期刊文献

英文题名：Microbiomics and metabolomics analysis reveals the unsynchronized growth mechanism in kuruma shrimp (Marsupenaeus japonicus)

作者：Zhao, Jichen[1,2];Liao, Minze[1,3];Zhu, Miao[1,2];Wang, Bo[1];Qin, Haipeng[1];Sun, Chengbo[1,4,5]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Peoples R China;[2]Guangdong Acad Agr Sci, Key Lab Anim Nutr & Feed Sci South China, Minist Agr & Rural Affairs, Inst Anim Sci,Guangdong Prov Key Lab Anim Breeding, Guangzhou 510640, Peoples R China;[3]Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Key Lab South China Sea Fishery Resources Exploita, Minist Agr & Rural Affairs, Guangzhou 510300, Peoples R China;[4]Guangdong Prov Lab Southern Marine Sci & Engn, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Key Lab Pathogen Biol & Epidemiol A, Zhanjiang 524088, Peoples R China

年份：2026

卷号：614

外文期刊名：AQUACULTURE

收录：SCI-EXPANDED(收录号：WOS:001638945100001)、、Scopus(收录号：2-s2.0-105023867216)、WOS

基金：This work was supported by Guangdong Basic and Applied Basic Research Foundation (2023A1515010117, 2023A1515011066) ; Guangzhou Science and Technology Planning Project (2024A04J3417) ; National Natural Science Foundation of China (32302977) .

语种：英文

外文关键词：Marsupenaeus japonicus; Unsynchronized growth; Microbiomics; Metabolomics; Growth biomarkers; Molecular mechanism

外文摘要：Unsynchronized growth represents a persistent challenge in crustacean aquaculture industry. While, the underlying growth mechanism of crustaceans remains poorly understood, which hinders the development of the breeding industry. This study employed microbiomics and metabolomics to analyze growth differences between fast growth group (FG) and slow growth group (SG) of the economically important crustacean kuruma shrimp (Marsupenaeus japonicus) across both fast and steady growth stages. Several differential metabolites (DMs) related to growth were identified and validated by ELISA. The microbiomics results showed that compared with the SG group, FG displayed lower Vibrio abundance (p > 0.05) coupled with enhanced carbohydrate metabolic capacity (p < 0.05), suggesting a potential role of the gut microbiota in nutrient utilization. LC-MS detected 2342 (positive mode) and 1811 (negative mode) muscle metabolites. DMs analysis revealed more metabolites elevated in SG versus FG groups across both growth stages and ionization modes. Specifically, SG showed 158/229 (fast growth stage) and 189/275 (steady growth stage) elevated metabolites in positive mode, and 142/149 (fast) and 72/85 (steady) in negative mode. Across growth stages, 75 (positive) and 30 (negative) metabolites showed persistent differential regulation (with same tendency). Among them, sixteen DMs in positive mode (e.g., Propionylglycine up arrow; Tolazoline down arrow) and six in negative mode (e.g., Pyruvate down arrow, Pantothenate down arrow) represent potential M. japonicus growth biomarkers. Centered on pyruvate metabolism, the pantothenate and CoA biosynthesis, glycolysis and citrate cycle, etc. are the key pathways regulating the growth of kuruma shrimp. The FG individuals may achieve growth superiority by prioritize pyruvate partitioning toward ATP/biosynthesis over storage-a key metabolic adaptation absent in SG groups. These findings refine models of crustacean growth modulation and advance precision aquaculture strategies.