文献类型：期刊文献

英文题名：Cytotoxicity and Apoptosis Induction of Bovine Alpha-lactalbumin-oleic Acid Complex in Human Breast Cancer Cells

作者：Zhong, Saiyi[1]; Liu, Shouchun[1]; Chen, Suhua[1]; Liu, Hai[1]; Zhou, Siyi[1]; Qin, Xiaoming[1]; Wang, Weimin[1];王维民[2];秦小明[2];

机构：Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Guangdong, Peoples R China; Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China; Guangdong Ocean Univ, Dept Expt Teaching, Modern Biochem Ctr, Zhanjiang 524088, Guangdong, Peoples R China

年份：2015

卷号：21

期号：1

起止页码：103

外文期刊名：FOOD SCIENCE AND TECHNOLOGY RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000349580200013)、、EI(收录号：20151700777436)、Scopus(收录号：2-s2.0-84928007899)、WOS

基金：This research project is supported by the grants from the Natural Science Foundation of Guangdong Province (No: S2012040006790), National Natural Science Foundation of China (No: 31201424) and the start-up fund from GDOU (E12319).

语种：英文

外文关键词：bovine alpha-lactalbumin-oleic acid; cytotoxicity; apoptosis; human breast cancer cells

外文摘要：Breast cancer is the mostly commonly diagnosed cancer in women worldwide. Using food-based approach to breast cancer chemoprevention has attracted a great deal of attention in recent years. In the present study, a-lactalbumin was purified fresh bovine milk and the complex with oleic acid (BLA-OA Complex) was prepared by chromatographic method. The cytotoxic and apoptotic activities of BLA-OA complex on human ERpositive MCF-7 and ER-negative MDA-MB-231 breast cancer cells was investigated by morphological observation, MTS assay, LDH release, DAPI staining, DNA ladder assay and cell cycle analysis. The results showed that BLA-OA complex inhibits cells growth and induces apoptosis in both human ER-positive MCF-7 and ER-negative MDA-MB-231 breast cancer cell lines. The findings of this study are positive for cow milk industry and human breast cancer chemoprevention.