文献类型：期刊文献

英文题名：Effects of a commercial probiotic BS containing Bacillus subtilis and Bacillus licheniformis on growth, immune response and disease resistance in Nile tilapia, Oreochromis niloticus

作者：Abarike, Emmanuel Delwin[1,2,3,5];Cai, Jia[1,2,3];Lu, Yishan[1,2,3];Yu, Huang[1,2,3];Chen, Lihua[4];Jian, Jichang[1,2,3];Tang, Jufen[1,2,3];Jun, Liang[4];Kuebutornye, Felix K. A.[1,2,3,5]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang 524088, Peoples R China;[3]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Anim, Zhanjiang 524088, Peoples R China;[4]Langye Anim Husb Co Ltd, Gaozhou City, Guangdong, Peoples R China;[5]Univ Dev Studies, Dept Fisheries & Aquat Resources Management, Tamale, Ghana

年份：2018

卷号：82

起止页码：229

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000447571600027)、、Scopus(收录号：2-s2.0-85051807706)、WOS

基金：The National Natural Science Foundation of China (31572651), Natural Science Foundation of Guangdong Province (2015A030308020) and (2016A030313748) and Science and Technology Planning Project of Guangdong Province (2015A020209181) supported this work.

语种：英文

外文关键词：Oreochromis niloticus; Streptococcus agalactiae; Growth; Gene expression; Bacillus

外文摘要：The present study evaluated a commercial probiotic designated as BS (a mix of B. subtilis and B. licheniformis) to ascertain its efficacy and the dose necessary to improve growth, immune response, and disease resistance in tilapia, Oreochromis niloticus. Fish (53.01 +/- 1.0 g) were fed with a basal diet supplemented with 0 gkg(-1) (CT), 3 gkg(-1) (BS3), 5 gkg-1 (BS5) 7 gkg-1 (BS7) and 10 gkg-1 (BS10) of the probiotic BS for 4 weeks. At the end of the feeding trial, the weight gain, specific growth rate, and feed conversion ration were enhanced in all probiotic BS enriched groups but with better (P < 0.05) improvement in the BS10 group. The lysozyme, protease, anti protease, superoxide dismutase activities, and immunoglobulin M level were significantly (P < 0.05) highest in the BS10 group in both serum and skin mucus. Enhanced (P > 0.05) catalse activity in all treated groups in the serum and myeloperoxidase activity in the B10 group in both serum and skin mucus were observed. The expression of C-lysozyme, heat shock protein 70, beta-defensin, transforming growth factor beta, and small body size decapentaplegic homolog 3, genes in the mid-intestines and the head-kidney were up-regulated in all treated groups with the BS10 group provoking the highest up-regulation (P < 0.05). After challenge with Streptococcus agalactiae, cumulative mortality was 80%, 47.5%, 42.8%, 30%, and 20% for fish fed with CT, BS3, BS5, BS7, BS10 groups respectively. In conclusion, probiotic BS application at 10 gkg(-1) (BS10) can be considered to improve growth and immunological status in tilapia farming.