文献类型：期刊文献

中文题名：尼罗罗非鱼核糖体蛋白S6基因克隆及组织表达分析

英文题名：Cloning and Tissue Expression Analysis of Ribosomal Protein S6 Gene in Nile Tilapia(Oreochromis niloticus)

作者：巫祎琴[1,2,3];张志强[1,2,3];陈福暖[1,2,3];江栢坚[1,2,3];简纪常[1,2,3];黄瑜[1,2,3]

机构：[1]广东海洋大学水产学院,湛江524088;[2]广东省水产动物病害防控与健康养殖重点实验室,湛江524088;[3]水产经济动物病害控制广东省普通高等学校重点实验室,湛江524088

年份：2022

卷号：41

期号：6

起止页码：1193

中文期刊名：基因组学与应用生物学

外文期刊名：Genomics and Applied Biology

收录：CSTPCD、、CSCD2021_2022、北大核心、CSCD、北大核心2020

基金：国家自然科学基金项目(32002426、32073006)共同资助

语种：中文

中文关键词：尼罗罗非鱼;核糖体蛋白S6;基因克隆;表达分析;无乳链球菌

外文关键词：Oreochromis niloticus;Ribosomal protein S6;Gene clone;Expression analysis;Streptococcus agalactiae

中文摘要：核糖体蛋白S6(ribosomal protein S6,RPS6)是构成40S小亚基核糖体的关键蛋白之一,在蛋白质合成、调控细胞周期和凋亡、调节肿瘤细胞增殖和转移等方面具有重要作用。为探究RPS6在尼罗罗非鱼(Oreochromis niloticus)中的分子结构、表达特性和生物学功能,本研究从尼罗罗非鱼中克隆获得RPS6基因的编码区序列(GenBank登录号:XM_003454192.4;命名为On-RPS6),并采用qPCR法分析该基因在健康鱼体各组织的分布特征以及无乳链球菌刺激后该基因在鱼体肝脏、脾脏、脑和头肾中的表达模式。结果显示:On-RPS6的开放阅读框长度为750 bp,编码249个氨基酸,理论分子量为28.70 kDa,等电点为10.90。氨基酸序列分析显示,On-RPS6具有一个高度保守的核糖体蛋白S6e结构域,且与其他物种具有较高同源性。系统进化树分析表明,尼罗罗非鱼RPS6与斑马拟丽鱼(Maylandia zebra)RPS6聚为一支,表明二者具有较近的亲缘关系。qPCR结果显示,尼罗罗非鱼各组织均能检测到On-RPS6基因mRNA的表达,且在血液中的表达量最高;经无乳链球菌刺激后,On-RPS6基因在肝脏、脾脏、脑和头肾中的表达模式均呈现先上调后下降的趋势。结果表明,On-RPS6在尼罗罗非鱼抵抗细菌感染的免疫应答过程中发挥了重要作用。

外文摘要：Ribosomal protein S6(RPS6),one component of the 40 S ribosomal subunit,plays an important roles in protein synthesis,regulation of cell cycle and apoptosis,tumor proliferation and metastasis in mammals.To explore the molecular structure,expression characteristics and biological function of RPS6 in Nile Tilapia(Oreochromis niloticus),in this study,the open reading frame of RPS6 gene was cloned from tilapia(GenBank accession number:XM_003454192.4;designated as On-RPS6),and the expression of On-RPS6 in healthy fish,and in liver,spleen,brain and head kidney of Streptococcus agalactis stimulated fish was analyzed by quantitative real-time PCR.The results showed that On-RPS6 was 750 bp in open reading frame,encoding 249 amino acids.The deduced amino acid sequence of On-RPS6 with an estimated molecular weight of 28.70 kDa and a theoretical pI of 10.90.Amino acid alignment indicated that On-RPS6 have a highly conserved region of ribosomal protein S6 e.Phylogenetic analysis showed that On-RPS6 was clustered closely with Maylandia zebra,which indicated that RPS6 of these two species had closely relationship.Moreover,the mRNA expression levels of On-RPS6 could be detected in all the examined tissues in healthy Nile tilapia and the highest expression level was be detected in the blood.When infected with Streptococcus agalactiae,the expression of On-RPS6 was significantly up-regulated in liver,spleen,brain and head kidney.In conclusion,On-RPS6 played crucial roles in the immune response of Nile tilapia against Streptococcus agalactis.