文献类型：期刊文献

英文题名：(-)-Gossypol-enriched Cottonseed Oil Inhibits Proliferation and Adipogenesis of Human Breast Pre-adipocytes

作者：Zhong, Saiyi[1];Leong, John[2];Ye, Weiping[2];Xu, Pingping[2];Lin, Shu-Hong[2];Liu, Jie-Yu[2];Lin, Young C.[2,3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Guangdong, Peoples R China;[2]Ohio State Univ, Coll Vet Med, Lab Reprod & Mol Endocrinol, Columbus, OH 43210 USA;[3]Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA

年份：2013

卷号：33

期号：3

起止页码：949

外文期刊名：ANTICANCER RESEARCH

收录：SCI-EXPANDED(收录号：WOS:000316770400026)、、Scopus(收录号：2-s2.0-84875874658)、WOS

基金：This research project is supported by the grants from the National Natural Science Foundation of China (No: 31201424), the Natural Science Foundation of Guangdong Province (No: S2012040006790) and the start-up fund from GDOU (E12319) to Dr Saiyi Zhong, and the grant from the Cotton Incorporated, Cary, NC, U.S.A. to Dr. Young C. Lin.

语种：英文

外文关键词：(-)-Gossypol-enriched cottonseed oil; proliferation; adipogenesis; human breast pre-adipocytes

外文摘要：Background: Breast cancer is the most commonly diagnosed cancer in women. Obesity is an important risk factor for developing breast cancer and is one of few risk factors that women can modify to prevent cancer. (-)Gossypol-enriched cottonseed oil [(-)-GPCSO] contains 65% (-)-gossypol and 35% (+)-gossypol. Previous studies have demonstrated that both (-)-gossypol and (-)-GPCSO have potent anticancer activity against multiple types of cancer, including breast cancer. In addition, (-)-GPCSO reduced body weight gain and food intake in young female rats. However, the role of (-)-GPCSO on adipogenesis in human breast pre-adipocytes remains unclear. Materials and Methods: Primary human breast pre-adipocytes were induced to differentiate in adipogenic medium in the presence of (-)-GPCSO. The proliferation of pre-adipocytes was determined with a 344,5-dime thylthiazol-2-yl)-5-(3-carb oxymethoxyphenyl)-2-(4-sulfophenyl)-2-H-tetrazolium (MTS) assay. Lipid accumulation and glycerol 3-phosphate dehydrogenase (GPDH) activity were measured during adipocyte differentiation. mRNA expression of cyclin-D1, B-cell lymphoma-2 (BCL-2), Peroxisome Proliferator-Activated Receptor-gamma (PPAR gamma), CCAAT/enhancer-binding protein alpha (CIEBP alpha) and leptin was analyzed by real-time PCR. Results: (-)-GPCSO inhibited proliferation of pre-adipocytes and down-regulated the expression of cyclin-D1 and BCL-2. (-)-GPCSO also significantly decreased adipo genesis, as determined by inhibition of GPDH activity, triglyceride content (TG), and down-regulation of the expression of PPAR gamma, CIEBP alpha and leptin. Conclusion: These findings suggest that (-)-GPCSO has the potential as a food supplement to inhibit adipogenesis, and therefore, reduce obesity.