文献类型：期刊文献

中文题名：硫酸黏菌素残留对土壤微生物群落结构特征的影响

英文题名：Effects of colistin sulfate residue on soil microbial community structure

作者：马驿[1];彭金菊[1];陈进军[1];范葶莉[1];孙永学[2]

机构：[1]广东海洋大学农学院动物医学系;[2]华南农业大学广东省兽药研制与安全评价重点实验室

年份：2014

卷号：25

期号：6

起止页码：1780

中文期刊名：应用生态学报

外文期刊名：Chinese Journal of Applied Ecology

收录：CSTPCD、、北大核心2011、Scopus、北大核心、CSCD、CSCD2013_2014、PubMed

基金：国家自然科学基金项目(31172368);广东省自然科学基金项目(S2012010010628)资助

语种：中文

中文关键词：硫酸黏菌素;土壤微生物生物量碳;群落结构;磷脂脂肪酸

外文关键词：colistin sulfate; soil microbial biomass carbon; community structure; phospholipid fat-ty acid.

中文摘要：采用氯仿熏蒸法和磷脂脂肪酸(phospholipid fatty acid,PLFA)方法,分析了硫酸黏菌素(colistin sulfate,CS)残留后土壤微生物群落结构的变化.结果表明:硫酸黏菌素(wCS≥5mg·kg-1)对土壤微生物生物量碳影响显著,土壤中硫酸黏菌素浓度愈高,微生物生物量碳愈低,50 mg·kg-1的硫酸黏菌素处理使土壤微生物生物量碳下降52.1%.在整个采样周期中,每克土壤总PLFA含量在硫酸黏菌素胁迫下出现明显降低,且存在一定的剂量依赖效应.第7、49天时,低浓度组(wCS=0.5 mg·kg-1)和对照组分异不明显,第21、35天时,处理间土壤微生物群落结构多样性类型分异显著,其中高浓度组(wCS=50 mg·kg-1)与对照组分异最大.表明硫酸黏菌素可致土壤微生物群落结构多样性改变,并表现出时间差异.这可能与硫酸黏菌素在土壤中化学结构发生了改变和降解有关.

外文摘要：By using fumigation extraction and phospholipid fatty acid （PLFA） methods, the change of characteristics of soil microbial community structure caused by residue of eolistin sulfate （CS） was studied. The results showed that the CS （ Wcs ≥5 mg · kg^-1 ） had a significant effect on the mi- crobial biomass carbon （MBC） and it was dose-dependent where MBC decreased with the increase of CS concentration in soil. The MBC in soil decreased by 52. 1% when the CS concentration reached 50 mg · kg^-1. The total PLFA of soil in each CS treatment was significantly decreased dur- ing the sampling period compared with the control group and showed a dose-dependent relationship. The soil microbial community structure and diversity in the low CS group （Wcs=0.5 mg · kg^-1） were not significantly different from the control group on 7th and 49th day. However, they were sig- nificantly different on 21st and 35th day especially in the high CS group （Wcs =50 mg · kg^-1）. It was concluded that CS could change the structure of soil microorganisms and varied with time which might be caused by the chemical conversion and degradation of CS in soil.