文献类型：期刊文献

中文题名：4种贝类糖胺聚糖体外清除自由基活性的比较

英文题名：Free radical-scavenging activity of glycosaminoglycans from four seashells in vitro

作者：范秀萍[1];吴红棉[1];王娅楠[1];黎少萍[1];胡雪琼[1]

机构：[1]广东海洋大学食品科技学院,湛江524006

年份：2008

卷号：33

期号：2

起止页码：165

中文期刊名：食品科技

外文期刊名：Food Science and Technology

收录：北大核心2004、北大核心

语种：中文

中文关键词：糖胺聚糖;自由基清除率;羟基自由基;超氧阴离子自由基

外文关键词：glycosaminoglycan（GAG）; radical scavenging ratio; hydroxyl radical; superoxide radical

中文摘要：目的:研究马氏珠母贝、菲律宾蛤仔、波纹巴非蛤和缢蛏4种贝类糖胺聚糖(GAG)体外对羟基自由基(HO·)和超氧阴离子自由基(O2-·)的清除作用。方法:采用邻二氮菲-Fe2+氧化法测定贝类糖胺聚糖对羟基自由基(HO·)的清除作用,采用邻苯三酚自氧化法检测超氧阴离子自由基(O2-·)的清除作用。结果:在羟基自由基体系中,波纹巴非蛤PUG显示较强的清除作用,IC50为6.75 mg/mL;马氏珠母贝CPG、菲律宾蛤仔CRG和缢蛏SG对羟基自由基的清除作用随剂量增加而上升,最高分别可达到47.9%、45.6%和45.9%;在超氧阴离子自由基体系中,马氏珠母贝CPG、菲律宾蛤仔CRG、波纹巴非蛤PUG、缢蛏SG的清除率最高分别可达到37.7%、36.8%、32.4%和36.5%。结论:3种贝类GAG在体外对羟基自由基与超氧阴离子自由基均具有一定的清除作用,显示一定的抗氧化活性。

外文摘要：Purpose： To study the free radical scavenging capacities of four kinds of crude products of glycosaminoglycans（GAG）. Methods： The scavenging effect to the hydroxyl radical were detected with the oxidation of 1,10-phenanthroline -Fe^2＋; the scavenging effect to the superoxide radical were determined with the self-oxidation of pyrogallic acid system, Results： In the hydroxyl radical assay system, GAG from Paphia undulata（PUG） showed good scavenging effect, IC50 was 6.45 mg/mL, While for GAG from Pinctada martensii （CPG）, Ruditapes philippinarum（CRG） and Sinonovacula constricta（SG）, free radical scavenging effects were as high as 47.9%, 45.6% and 45.9% respectively. In the superoxide radical assay system, the scavenging ratio of CPG, CRG, PUG and SG were as high as 37.7%, 36.8%, 32.4% and 36.5% respectively. Conclusion： The four kinds of glycosaminoglycans have certain scavenging effect to hydroxyl radical and superoxide radical.