文献类型：期刊文献

中文题名：飞机草提取物对斑马鱼心、脑毒性的病理组织学影响

英文题名：Histopathological Changes of Heart and Brain in Zebrafish Exposure to Eupatorium odoratum Extract

作者：张孝敏[1];陈柳红[1];郑润凯[1];张绮玲[1];林红英[1];陈进军[1]

机构：[1]广东海洋大学农学院,广东湛江524088

年份：2010

卷号：30

期号：1

起止页码：27

中文期刊名：广东海洋大学学报

外文期刊名：Journal of Guangdong Ocean University

收录：CSTPCD

基金：广东海洋大学大学生研究创新性实验项目(校教务〔2009〕3号)

语种：中文

中文关键词：飞机草提取物;病理组织学;心;脑;斑马鱼

外文关键词：Eupatorium odoratum Extract; histopathological change; heart, brain;zebrafish （Danio rerio）

中文摘要：制备飞机草95%(φ)乙醇提取物冻干粉,以200、500、800mg/L3种用量对斑马鱼进行水浴染毒,21d后取斑马鱼的心和脑制作病理组织切片,显微观察。结果表明,提取物冻干粉导致斑马鱼心肌细胞溶解,萎缩,严重时坏死,脑组织局灶性炎性细胞浸润、血栓形成和各细胞层结构变化,随着提取物冻干粉含量的增大,斑马鱼心、脑的病理组织学变化更加明显。乙醇提取物的冻干粉对斑马鱼的心、脑具有较大的毒性作用。

外文摘要：The frozen powder of Eupatorium odoratum extract was prepared with whose volume faction is 95% and the extract from different concentrations was exposed to the groups of zebrafish （Danio rerio）, respectively. On the 21th day of exposure, the heart and brain were collected, respectively and the histopathological changes were observed. It was found that the E. odoratum extract caused toxic effects in heart of the zebrafish as dissolution, atrophy and necroses of cardiac cells, and that the brain tissue was invaded with inflammatory cells, thrombosis and structural changes in the cell layers. In addition, the higher the concentration of the E. odoratum extract, the greater the histopathological changes in heart and brain showed. It was concluded that the cardiovascular system and the central nervous system of the zebrafish had higher sensitivity to the E. odoratum extract.