文献类型：期刊文献

英文题名：Molecular Cloning and Expression Analysis of IgD in Nile Tilapia (Oreochromis niloticus) in Response to Streptococcus agalactiae Stimulus

作者：Wang, Bei[1];Wang, Pei[2];Wu, Zao-He[1];Lu, Yi-Shan[1];Wang, Zhong-Liang[1];Jian, Ji-Chang[1]

机构：[1]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Guangdong Key Lab Control Dis Aquat Econ Anim, Zhanjiang 524088, Peoples R China;[2]Guangxi Key Lab Beibu Gulf Marine Biodivers Conse, Qinzhou 535099, Peoples R China

年份：2016

卷号：17

期号：3

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：SCI-EXPANDED(收录号：WOS:000373712800045)、、WOS

基金：This work was supported by National Natural Science Foundation of China (Grant No. 31302226), Science & Technology Planning Project of Guangdong Province of China (Grant No. 2013B020307014), Guangdong Ocean University Funds for Distinguished Young Scientists (Grant No. HDYQ2015001) and Guangxi Key Laboratory of Beibu Gulf Marine Biodiversity Conservation, Qinzhou University (Grant No. 2015KB05). We are especially grateful for the critical comments from the anonymous reviewers.

语种：英文

外文关键词：Streptococcus agalactiae; IgD; fluorescent quantitative real-time PCR; Oreochromis niloticus

外文摘要：IgD is considered to be a recently-evolved Ig and a puzzling molecule, being previously found in all vertebrate taxa, except for birds. Although IgD likely plays an important role in vertebrate immune responses, the function of IgD in Nile tilapia (Oreochromis niloticus) is virtually unknown. In the present study, a membrane form of IgD (mIgD) heavy chains were cloned from the GIFT strain of Nile tilapia (designated On-mIgD). The On-mIgD heavy chain's cDNA is composed of 3347 bp with a 31 bp of 5 '-UTR, 3015 bp open reading frame (ORF) and 301 bp 3 '-UTR, encoding a polypeptide of 1004 amino acids (GenBank accession no: KF530821). Phylogenetic analysis revealed that On-mIgD heavy chains showed the highest similarity to Siniperca chuatsi. Quantitative real-time PCR (qRT-PCR) analysis showed that On-mIgD expression occurred predominately in head kidney, thymus, spleen, and kidney. After Streptococcus agalactiae infection, transcripts of On-mIgD increased and reached its peak at 48 h in the head kidney and thymus, and 72 h in the spleen, respectively. Taken together, these results collectively indicated that IgD could possibly have a key role to play in the immune response when bacterial infections in Nile tilapia.