文献类型：期刊文献

英文题名：Immunoproteomic analysis and identification of novel immunogenic proteins from Vibrio harveyi

作者：Pang, H. -Y.[1,2,3,4,5];Li, Y.[1,4,5];Wu, Z. -H.[1,4,5];Jian, J. -C.[1,4,5];Lu, Y. -S.[1,4,5];Cai, S. -H.[1,4,5]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang City 524025, Guangdong, Peoples R China;[2]Chinese Acad Sci, S China Sea Inst Oceanol, Guangzhou, Guangdong, Peoples R China;[3]Chinese Acad Sci, Grad Sch, Beijing, Peoples R China;[4]Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China;[5]Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China

年份：2010

卷号：109

期号：5

起止页码：1800

外文期刊名：JOURNAL OF APPLIED MICROBIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000282813800031)、、Scopus(收录号：2-s2.0-78650468000)、WOS

基金：We thank Dr Phillip Klesius (USAD, ARS, Aquatic Animal Health Research laboratory, Auburn, Al, USA) for his critical review of the manuscript. This work was supported by research grants (No. 30972271) from the National Natural Science Foundation of China.

语种：英文

外文关键词：immunoproteome; Vibrio harveyi; whole-cell protein

外文摘要：Aims: The main aim of this study was to screen novel immunogenic proteins of Vibrio harveyi, which could be vaccine candidates. Methods and Results: Whole-cell proteins of V. harveyi, strain Li01 and Huang01, were first separated by isoelectric focusing, followed by 2D-PAGE, respectively. Immunogenic proteins were identified by Western blotting, using Epinephelus coioides antisera against V. harveyi strain Li01. Western blot analyses revealed 16 shared immunogenic protein spots in both strains. All of the immunogenic proteins were successfully identified and corresponded to 15 proteins. None of these proteins have been previously reported as immunogenic for V. harveyi. Of the 15 proteins, 11 are specific immunoreactive proteins and four are nonspecific immunoreactive proteins. Furthermore, outer membrane protein N (spot 2) and oligopeptide ATP-binding cassette (ABC) transporter (spot 3) were used as immunogens to immunize E. coioides for investigation of their protective abilities and activities. The E. coioides immunized with OmpN has abilities to fight against infections caused by V. harveyi Li01 and Huang01. However, vaccination with oligopeptide ABC transporter induces low protective immune response in fish. Conclusions: Eleven novel specific antigens were found, and OmpN could potentially be used as vaccine candidate for the development of novel vaccine against V. harveyi. Significance and Impact of the Study: These data show that immunoproteomics methods can be successfully applied in identifying immunogenic proteins of V. harveyi, which helps to search for the protective antigens in future.