文献类型：期刊文献

英文题名：Insights into the response mechanism of Litopenaeus vannamei exposed to cold stress during live transport combining untargeted metabolomics and biochemical assays

作者：Xu, Defeng[1,2];Zheng, Xiaoxian[1];Li, Caihong[3];Wu, Jiaxin[1];Sun, Lijun[1];Qin, Xiaoming[1];Fan, Xiuping[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China;[3]Guangdong Med Univ, Sch Basic Med Sci, Dongguan 523808, Guangdong, Peoples R China

年份：2022

卷号：104

外文期刊名：JOURNAL OF THERMAL BIOLOGY

收录：SCI-EXPANDED(收录号：WOS:000761258400005)、、WOS

基金：The authors gratefully acknowledge the National Natural Science Foundation from China (31772048), the National Key Research and Development Program of China (2019YFD0901601), and the Funds for PHD researchers of Guangdong Medical University in 2017 (B2017038), the Natural Science Foundation of PHD researchers of Guangdong Province in 2018 (2018A030310111) for the financial support.

语种：英文

外文摘要：Unfavorable conditions severely affect the survival quality of Litopenaeus vannamei (L. vannamei) during live transport and the molecular response mechanism needs to be clarified. In this study, metabolomics combined with conventional assay on physiological and histopathological responses were applied to deepen the understanding of L. vannamei to cold stress and provide. A solid foundation for regulation of transportation management. Physiological and biochemical analysis revealed the significant disturbance of glycolysis in hepatopancreas tissue. Furthermore, metabolomics based on the technique of ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry identified the significantly differential metabolites between acute cold exposure (AC) and normal control (NC) groups. Moreover, KEEG result indicated that the pathways of amino acid, glycerophospholipid, and nuclei acid metabolism and ABC transporters in hepatopancreas were significantly disturbed. Furthermore, histopathology and ultrastructure verified the impairment in hepatopancreas during cold stress. Overall, the concurrent use of metabolomics and biochemical assays was demonstrated to be sensitive and effective in providing new insights into the response mechanism of L. vannamei to cold stress.