文献类型：期刊文献

中文题名：不同组分马尾藻岩藻聚糖硫酸酯对人肺癌细胞A549作用的研究

英文题名：Effect of different components of fucoidan from Sargassum siliquastrum on Lung Cancer A549 Cells

作者：蔡璐[1];王维民[1];谌素华[1];丁伟[2];李春莲[1]

机构：[1]广东海洋大学食品科技学院,广东湛江524088;[2]旭骏水产(湛江)有限公司,广东湛江524057

年份：2014

卷号：35

期号：1

起止页码：116

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、北大核心2011、北大核心、CSCD、CSCD2013_2014

基金：广东省科技计划项目(2011B20310010)

语种：中文

中文关键词：马尾藻岩藻聚糖;人肺癌细胞A549;抑制效果;铺板

外文关键词：fucoidan from Sargassum siliquastrum; Lung Cancer A549 Cells; inhibitory effect ~ planking

中文摘要：本研究采用不同组分马尾藻岩藻聚糖硫酸酯（sF）对人肺癌细胞A549细胞的抑制作用进行了研究。根据Mtt比色法检测不同细胞铺板数量及不同质量浓度sF对该细胞生长曲线的影响；不同组分sF在不同时间对该细胞的增殖抑制率；倒置显微镜下观察SF对该细胞形态的影响。研究结果表明，当SF的浓度在1-25mg／mL范围、细胞铺板数量为5×10个／mL时，细胞生长曲线线性良好；各组分sF对人肺癌细胞A549均有抑制作用，其中SF2一R对细胞的抑制效果较强，抑制率为61．06％。

外文摘要：In this study,the effect of sargasso fucosan sulfate（SF） with different components on inhibition of human lung cancer A549 cells was studied. The effect of different cell counts in the planking and SF with different concentrations on cell growth curve was detected according to the MTT assay;the proliferation inhibition rate of SF with different components on this cell at different times;the effect of SF on morphology of this cell was observed under an inverted microscope.The results showed that,when concentration of SF was set in the range of 1-25mg/mL,cell count in the planking was 5 x ]03 cell/mL, the cell growth curve had a good linearity;SF with different components all had inhibitory effect on human lung cancer A549 cells,in which SF2-R had the strongest inhibitory effect on the cells, and the inhibition rate was 61.06%.