文献类型：期刊文献

英文题名：Basic fibroblast growth factor upregulates expression of growth hormone gene through extracellular signal-regulated kinase 1/2 in GH4 cells

作者：Liu, H. Z.[1];Luo, P.[2];Chen, S. H.[1];Shang, J. H.[3]

机构：[1]Guangdong Ocean Univ, Modem Biochem Ctr, Zhanjiang, Peoples R China;[2]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang, Peoples R China;[3]Chinese Acad Agr Sci, Buffalo Res Inst, Guangxi Key Lab Buffalo Genet Reprod & Breeding, Nanning 530001, Peoples R China

年份：2013

卷号：114

期号：7

起止页码：376

外文期刊名：BRATISLAVA MEDICAL JOURNAL-BRATISLAVSKE LEKARSKE LISTY

收录：SCI-EXPANDED(收录号：WOS:000322290300003)、、Scopus(收录号：2-s2.0-84880950924)、WOS

基金：This work was supported by Natural Science Foundation of Guangxi Zhuang Autonomous Region of China (0991011) and the Opening Project of Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding (SNKF-2011-02).

语种：英文

外文关键词：basic fibroblast growth factor; growth hormone; ERK1/2; GH4 cells

外文摘要：Basic fibroblast growth factor (bFGF) is reported to not only play multifunctions in pituitary differentiation and tumor formation, stimulating cell differentiation or proliferation, also stimulate pituitary to secret prolactin, growth hormone (GH) and thyroid stimulating hormone, although obvious effect on growth hormone only responded to high-dose bFGF. Since it is well documented that both bFGF and GH correlate closely to tumorigenesis, development and metastasis, so it is necessary to reveal the relationship between the cytokines. In the present report we investigated the effect of bFGF on transcription level of GH gene in GH4 rat pituitary cells as well as the regulatory mechanism with real-time reverse transcription polymerase chain reaction and western blotting. We observed a significant expressional increase of GH gene in GH4 cells stimulated by bFGF, meanwhile phosphorylation of extracellular signal-regulated kinase 1/2 (ERK1/2) was also found in the cells. Further investigation unveiled that PD98059, a specific inhibitor of ERK1/2 signaling pathway markedly impaired the transcriptional increment of GH gene induced by bFGF in the pituitary cells, which indicates that bFGF upregulates OH gene expression through ERK1/2 signaling pathway in GH4 cells. Results may be helpful to elaborate roles of the two cytokines on tumor (Fig. 3, Ref. 25). Full Text in PDF www.elis.sk.