文献类型：期刊文献

英文题名：A sensitive method for simultaneous quantitative determination of surfactin and iturin by LC-MS/MS

作者：Deng, Qi[1];Wang, Wenjing[1];Sun, Lijun[1];Wang, Yaling[1];Liao, Jianmeng[2];Xu, Defeng[1];Liu, Ying[1];Ye, Riying[1];Gooneratne, Ravi[3]

机构：[1]Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Key Lab Adv Proc Aquat Prod, Guangdong Higher Educ Inst,Coll Food Sci & Techno, 1 Haida Rd, Zhanjiang 524088, Guangdong, Peoples R China;[2]Natl Marine Prod Qual Supervis & Inspect Ctr, Zhanjiang 524000, Guangdong, Peoples R China;[3]Lincoln Univ, Ctr Food Res & Innovat, Dept Wine Food & Mol Biosci, Canterbury 7647, New Zealand

年份：2017

卷号：409

期号：1

起止页码：179

外文期刊名：ANALYTICAL AND BIOANALYTICAL CHEMISTRY

收录：SCI-EXPANDED(收录号：WOS:000391357700019)、、EI(收录号：20164302942230)、Scopus(收录号：2-s2.0-84991822147)、WOS

基金：The authors gratefully acknowledge the public research and capacity building program of Guangdong Province (Nos. 2014B020204005 and 2014B020205006) and the higher educational cultivation program for major scientific research projects of Guangdong Ocean University (Nos. GDOU2013050205, 2014050203, 2013050312) for their financial support.

语种：英文

外文关键词：LC-MS/MS; Antimicrobial lipopeptide; Bacillus natto; Surfactin; Iturin

外文摘要：Surfactin and iturin are antimicrobial lipopeptides produced from Bacillus spp. and have significant prospective applications in many fields. Therefore, accurate analysis of these lipopeptides in the fermented product of some Bacillus strains is important. A sensitive method for simultaneous quantitative determination of surfactin and iturin fermented by Bacillus natto NT-6 was developed and validated using liquid chromatography-tandem mass spectrometry. Crude extracts of antimicrobial lipopeptide samples were dissolved in a mixture of acetonitrile/water (7:3, v/v) in 0.1 % (v/v) formic acid and eluted with acetonitrile/water (7:3, v/v) containing 5 mmol L-1 ammonium acetate and 0.1 % (v/v) formic acid. The target compounds were detected by mass spectrometry (ESI+) using selective ion monitoring. A good linear regression in the range of 0.20-10.0 mg L-1 for both surfactin and iturin (R-2 >= 0.9995) was observed with spiked recoveries of 93.3-108.2 %, RSD values less than 15 %, precision 4.14-13.30 %, and a detection limit of 0.374 mg L-1. This method has a simple preprocessing operation, good repeatability, and provides an accurate quantitative analysis of surfactin and iturin.