文献类型：期刊文献

中文题名：草莓离体培养中玻璃化的发生及克服措施研究

英文题名：Vitrification in in Vitro Culture of Strawberry and Its Prevention

作者：丰锋[1];陈厝边[1]

机构：[1]广东海洋大学农学院,广东湛江524088

年份：2007

卷号：29

期号：4

起止页码：78

中文期刊名：西南大学学报（自然科学版）

外文期刊名：Journal of Southwest University(Natural Science Edition)

收录：CSTPCD、、北大核心2004、CSCD_E2011_2012、北大核心、CSCD

基金：广东省农业厅农业科技项目(B06026)

语种：中文

中文关键词：草莓;离体培养;玻璃化

外文关键词：strawberry; in vitro culture; vitrification

中文摘要：以“丰香”品种为试材,MS为基本培养基,研究草莓玻璃化的克服措施,结果表明,在6-BA试验浓度范围内(1-3 mg.L-1),玻璃化率随6-BA浓度的增加而增加;40 g.L-1蔗糖,降低MS基本培养基中铵离子浓度,加入活性炭,采用透气膜封口在一定程度上均能使玻璃苗恢复正常.

外文摘要：Cultured in vitro on MS medium supplemented with 6-BA, the strawberry variety ＂Tochiotome＂ showed increasing vitrification rate with increasing 6-BA concentration （1-3 mg/L）. The addition of su- crose to the medium at 40 g/L helped to decrease the number of vitreous plantlets. The measures of decreasing NH＋ concentration in the medium, or adding active carbon to it, or sealing the bottle with a permeable film helped the vitreous plantlets change back to normal ones.