文献类型：期刊文献

中文题名：鲎血细胞蛋白营养学评价及其水解肽体外活性

英文题名：Nutritional Evaluation of Blood-cell Protein from Tachypleus tridentatus and Activities of Its Hydrolysate in Vitro

作者：邓春梅[1];方少杏[1];何兰珍[1];张国光[1];吴育廉[1];洪鹏志[2];康信煌[1]

机构：[1]广东海洋大学化学与环境学院,广东湛江524088;[2]广东海洋大学食品科技学院,广东湛江524088

年份：2018

卷号：39

期号：21

起止页码：252

中文期刊名：食品工业科技

外文期刊名：Science and Technology of Food Industry

收录：CSTPCD、、CSCD_E2017_2018、北大核心2017、北大核心、CSCD

基金：广东海洋大学自然科学基金(Q15094;E13503;Q14176;2013A090100009);湛江市科技局自然科学基金(A15445;A14457;2016A02023);863国家高技术研究发展计划(2013AA102201)

语种：中文

中文关键词：鲎;血细胞蛋白;营养学评价;抗氧化;酶抑制

外文关键词：Tachypleus tridentatus;blood- cell protein;nutritional evaluation;antioxidation;enzymic inhibition

中文摘要：对鲎血细胞蛋白(TTBCP)进行营养学评价,并测定TTBCP水解物的体外抗氧化性、α-葡萄糖苷酶和乙酰胆碱酯酶的抑制活性。结果显示:TTBCP中必需氨基酸优于成人的FAO/WHO推荐模式,基本符合小孩的FAO/WHO推荐模式,其中必需氨基酸与总氨基酸的比值(E/T)为31.2%,氨基酸评分(AAS)为68.1,蛋白质效率比(PER)小于2.0。TTBCP水解肽对α-葡萄糖苷酶的半数抑制浓度(IC_(50))为0.199 mg/mL,抑制率最高达到83.8%,对乙酰胆碱酯酶同样有抑制作用;TTBCP水解肽能清除超氧阴离子自由基、DPPH自由基和羟自由基,当浓度小于0.75 mg/mL的范围时,还原能力大于维生素C。由此可见,TTBCP具有复合功效,可作为糖尿病的保健食品和药品开发利用。

外文摘要：The nutritional evaluation of blood - cell protein from Tachypleus tridentatus （ TTBCP ） was carried out, and its antioxidant activities, the inhibitory activities on a-glucosidase and acetylcholinesterase were measured in vitro. The results showed that the essential amino acids in TrBCP were superior to the adult＇ s FAO/WHO- recommending model, basically conforming to the child＇ s FAO/WHO-recommendating model.The ratio of essential amino acids to total amino acids（E/T） was 31.2% ,the amino acid score （AAS）was 68.1, the protein efficiency ratio （PER）was less than 2.0. The half inhibitory concentration（ ICs0 ） of TTBCP on c^-glucosidase was 0.199 mg/mL with the highest inhibition rate of 83.8 mg/mL.TTBCP also had inhibitory effect on cholinesterase.The hydrolysate peptide of TTBCP could scavenge the superoxide anion radicals, DPPH radicals and hydroxyl radicals,its reduction ability was greater than vitamin C when the concentration was less than 0.75 mg/mL. So, TTBCP could be developed into a health food and medicine for diabetes mellitus owing to its comprehensive efficacy.