文献类型：期刊文献

英文题名：Protection against Vibrio alginolyticus in pearl gentian grouper (female Epinephelus fuscoguttatus x male Epinephelus lanceolatu) immunized with an acfA-deletion live attenuated vaccine

作者：Chen, Yanyan;Cai, Shuanghu[1];Jian, Jichang[1]

机构：[1]Guangdong Ocean Univ, Coll Fisheries, Guangdong Prov Key Lab Pathogen Biol & Epidemiol, Zhanjiang, Peoples R China; Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Econ Anim, Zhanjiang, Peoples R China

年份：2019

卷号：86

起止页码：875

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000459528400095)、、WOS

基金：This work was supported by the National Natural Science Foundation of China (No. 31572656), Natural Science Foundation of Guangdong Province (No. 2015A030308020 and 2017A030307033), and Highlevel Talents Project of Sailing Plan in Guangdong Province.

语种：英文

外文关键词：Vibrio alginolyticus; acfA gene; Attenuated live vaccine; Pearl gentian grouper

外文摘要：Vibrio alginolyticus is well-known as an opportunistic Grain-negative pathogen, which endangers the development of global aquaculture as well as human health. In this study, a Delta acfA mutant strain and complementation of the Delta acfA mutant (C-acfA) were constructed. The Delta acfA mutant was tested in pearl gentian grouper (female Epinephelus fuscoguttatus x male Epinephelus lanceolatu) to observe the changes in virulence and evaluate its potential as an attenuated live vaccine. The results showed that the Delta acfA mutant caused a high antibody titer and a significant reduction in the ability to colonize the intestine of pearl gentian grouper. Grouper vaccinated with Delta acfA mutant were more tolerant of the infection by virulent V. alginolyticus HY9901 without inducing clinical symptoms and obvious pathological changes. The relative percent survival value of pearl gentian grouper vaccinated with Delta acfA mutant intraperitoneal injection reached 81.1% after challenging with V. alginolyticus HY9901. The specific antibody titers immunized with Delta acfA was significantly higher than that in the PBS group. The antibody titer of Delta acfA group displayed the tendency of rising up from the first to fourth week and declining from fifth to eighth week and reached the peak at the fourth week. In the meanwhile, the expression level of genes associated with immunity, including IL-1 beta, TNF-alpha, IL-16, IgM, CD8 alpha and MHC-I alpha, was up-regulated after vaccination, indicating that the Delta acfA can induce effective and durable immune response in pearl gentian grouper and it may be an effective attenuated live vaccine candidate for the prevention of infections by V. alginolyticus.