文献类型：期刊文献

英文题名：Construction of a growth model in the green alga Tetraselmis subcordiformis using a response surface approach

作者：Wang, Hui[1];Niu Jingyan[2];Liu Jiahui[3];Yang Hongshuai[3];Liu Zhigang[3]

机构：[1]Huaiyin Normal Univ, Life Sci Coll, Huaian 223300, Peoples R China;[2]Jiaozuo Teachers Coll, Polytech Dept, Jiaozuo 454000, Peoples R China;[3]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524025, Peoples R China

年份：2017

卷号：35

期号：3

起止页码：537

外文期刊名：CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000399168400007)、、WOS

基金：Supported by the Guangdong Province Science and Technology Project (No. 2010B020201014), the Guangdong Province South China Sea Invertebrates Healthy Culture Engineering Technology Research Center (No. GCZX-A0909), the Guangdong Province Ocean and Fisheries Science and Technology Extension Project (No. 20120980), the Guangdong Province Industry-University-Science Partnership Project (No. 20110908), and the Sci & Tech Plan of Huaiyin Normal University (No. WH0031)

语种：英文

外文关键词：Tetraselmis subcordiformis; specific growth; temperature; salinity; pH; growth model

外文摘要：The green alga Tetraselmis subcordiformis has been widely used as a quality live food for aquaculture species, and also has been studied as a model organism for the photo-biological production of hydrogen. We attempted to quantify the relationship between T. subcordiformis specific growth rate (SGR) and three important environmental factors (temperature, salinity, and pH) using the central composite design and response surface method under laboratory conditions. The results showed that the linear effects of temperature and salinity were significant (P < 0.05), and they were equally important in impacting T. subcordiformis specific growth; the linear effect of pH was not significant (P > 0.05); the interactive effect of temperature and pH was significant (P < 0.05), whereas the temperature x salinity and salinity x pH interactions were not significant (P > 0.05); all of the quadratic effects of the three factors were significant (P < 0.05). A model equation for specific growth rate with the three factors was established, with the unadjusted and predictive R-2 as high as 0.990 and 0.921, respectively, suggesting that the model was a very good fit and that it could be used to predict SGR. Through optimizing the reliable model, an optimal 3-factor combination of 25 A degrees C/35 of salinity/pH 7.9 was obtained, at which the maximum specific growth rate (0.65) was recorded, with a desirability value of 93.8%. These experimental results could serve as guidelines for increasing T. subcordiformis production efficiency.