文献类型：期刊文献

英文题名：Computational screening for novel α-glucosidase inhibitory peptides from Chlamys nobilis adductor muscle as a potential antidiabetic agent

作者：Lin, Haisheng[1];Wang, Wen[1];Du, Lei[2];Gao, Jialong[1];Cao, Wenhong[1];Zheng, Huina[1];Chen, Zhongqin[1];Qin, Xiaoming[1];Liang, Yuanwei[3]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Natl Res & Dev Branch Ctr Shellfish Proc, Guangdong Prov Key Lab Aquat Prod Proc & Safety, Zhanjiang, Peoples R China;[2]East China Univ Sci & Technol, Dept Food Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[3]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang, Peoples R China

年份：2025

卷号：12

外文期刊名：FRONTIERS IN NUTRITION

收录：SCI-EXPANDED(收录号：WOS:001460178200001)、、Scopus(收录号：2-s2.0-105001848276)、WOS

基金：The author(s) declare that financial support was received for the research and/or publication of this article. This work was supported by the earmarked fund for project supported by the National Key Research and Development Program (2024YFD2401903), the earmarked fund for the China Agriculture Research System (CARS-49), the Guangdong Province Modern Agricultural Industry Technology System Innovation Team Construction Project (2023KJ146), and the Open Project Program of Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety (GDPKLAPPS2001).

语种：英文

外文关键词：Chlamys nobilis; alpha-Glucosidase inhibitory peptides; molecular docking; glucose tolerance; antioxidant activity

外文摘要：Introduction This study aimed to evaluate the adjuvant hypoglycemic function of enzyme hydrolyzate (EHCA) from Chlamys nobilis in mice and to identify alpha-glucosidase inhibitory peptides.Methods The alpha-glucosidase inhibitory and radical scavenging ability of EHCA were determined in vitro, and the effects on blood glucose regulation and the antioxidant activity were evaluated in vivo using a mouse model. Peptides with potential alpha-glucosidase inhibitory activity were identified by LC-MS/MS and confirmed in silico.Results and Discussion EHCA exhibited significant alpha-glucosidase inhibitory activity and radical scavenging activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH). in vivo, EHCA significantly improved the glucose tolerance of mice, reduced malondialdehyde and increased the superoxide dismutase activity in liver. Five novel peptides were identified, with Lys-Leu-Asn-Ser-Thr-Thr-Glu-Lys-Leu-Glu-Glu and Thr-Asp-Ala-Asp-His-Lys-Phe showing strong inhibitory effects on alpha-glucosidase (IC50 value of 144.89 mu M and 136.96 mu M, respectively). The interactions between peptides and alpha-glucosidase were driven by hydrogen bonds, van der Waals forces, and hydrophobic interactions. These findings suggest that EHCA and its derived peptides could serve as potential adjuvant agents for blood glucose regulation and antioxidant activity. The identified peptides may pave the way for the development of alternative alpha-glucosidase inhibitors.