文献类型：期刊文献

英文题名：Antibacterial mechanism of slightly acidic electrolyzed water against Pseudomonas arsenicoxydans A1 from greater amberjack (Seriola dumerili)

作者：Zhong, Hongliang[1];Liu, Yinglin[1];Wei, Shuai[1,2];Khan, Imran[3];Sun, Qinxiu[1];Wang, Zefu[1];Han, Zongyuan[1];Liu, Yang[1];Oh, Deog-Hwan[4];Liu, Shucheng[1,2]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Key Lab Aqu, Guangdong Prov Engn Lab Marine Biol Prod, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China;[3]Univ Haripur, Dept Food Sci & Technol, Haripur 22620, Pakistan;[4]Kangwon Natl Univ, Dept Food Sci & Biotechnol, Coll Agr & Life Sci, Chunchon 200701, South Korea

年份：2024

卷号：194

外文期刊名：LWT-FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:001174040700001)、、EI(收录号：20240615509385)、Scopus(收录号：2-s2.0-85184042401)、WOS

基金：This research was supported by Special Fund for Scientific and Technological Innovation Strategy of Guangdong Province (2022A05036), Guangdong Innovation Team of Seafood Green Processing Technology (2019KCXTD011), Scientific Research Start-Up Funds of Guangdong Ocean University (R20048), and Innovation Team Program of Guangdong Ocean University (CXTD2024004).

语种：英文

外文关键词：Non -thermal treatment; Seafood; Spoilage bacteria; Inhibition

外文摘要：This study aimed to identify the specific spoilage organism (SSO) from the metamorphosed greater amberjack (Seriola dumerili) fish. Pseudomonas arsenicoxydans A1 was identified as SSO by culture-depending method. Further, the antibacterial efficacy of slightly acidic electrolyzed water (SAEW) was explored and the mechanism of action on P. arsenicoxydans A1 was studied. SAEW with 20-60 mg/L of available chlorine concentration (ACC) showed a good antibacterial effect on P. arsenicoxydans A1. The total number of colonies, growth curve, OD value at 260 nm and 280 nm, DNA fluorescence intensity, alkaline phosphatase (AKP), lactate dehydrogenase (LDH), superoxide dismutase (SOD), and catalase (CAT) enzyme activity and SEM results showed that SAEW could destroy the permeability and integrity of P. arsenicoxydans A1 cell membrane, and affect bacterial cell activity at metabolic, enzymatic and DNA level, which caused bacterial cell death. This study provided the application of SAEW in sterilization and preservation of raw fish fillets, which showed a promising effect on the growth of P. arsenicoxydans A1 in food cold chain.