文献类型：期刊文献

中文题名：4种笛鲷的线粒体DNA多样性分析

英文题名：Diversity analysis of mtDNA in the four species of snappers(Lutjanus)

作者：肖翔[1];刘楚吾[1]

机构：[1]湛江海洋大学海洋生物研究所,广东湛江524025

年份：2006

卷号：30

期号：4

起止页码：59

中文期刊名：海洋科学

外文期刊名：Marine Sciences

收录：CSTPCD、、北大核心2004、CSCD2011_2012、北大核心、CSCD

基金：广东省科技计划项目重大专项(A3050202);湛江市科技攻关项目(200017)

语种：中文

中文关键词：笛鲷;遗传多样性;mtDNA

外文关键词：Lutjanus; genetic diversity; mtDNA

中文摘要：取红鳍笛鲷(Lutjanus erythopterusBloch)、紫红笛鲷(Lutjanus argentimaculatusFor-skal)、勒氏笛鲷(Lutjanus russelliBleeker)和约氏笛鲷(Lutjanus johniBloch)肝脏组织,分离纯化其线粒体DNA,用限制性内切酶酶切进行了限制性片段长度多态性分析。在红鳍笛鲷共发现4种单倍型,单倍型间的平均遗传距离为0.010 1,其mtDNA多态度为0.002 9。在紫红笛鲷共发现6种单倍型,单倍型间的平均遗传距离为0.015 1,其mtDNA多态度为0.005 5。在勒氏笛鲷共发现3种单倍型,单倍型间的平均遗传距离为0.008 6,其mtDNA多态度为0.001 2。在约氏笛鲷共发现5种单倍型,单倍型间的平均遗传距离为0.008 3,其mtDNA多态度为0.002 0。

外文摘要：We used mtDNA-RFLP technique to analyse the genetic diversity of 4 species of snappers, Lutjanus erythopterus, Lutjanus argentimaculatus , Lutjanus russelli and Lutjanu johni. The mitochondrial DNAs （mtDNAs） from livers of these fishes were isolated by means of differential centrifugation and nuclease digestion followed by phenol extraction. The mtDNAs were digested by 19 varieties of restriction enzymes （5 or 6 base pairs） ： BarnH Ⅰ, Bgl Ⅰ, Bgl Ⅱ, EcoR Ⅰ, EcoR Ⅴ, Hind Ⅲ, Hinf Ⅰ, Hpa Ⅰ, Kpn Ⅰ, MluⅠ, Pst Ⅰ, Pvu Ⅱ, Sal Ⅰ, Sca Ⅰ, Srna Ⅰ, Sty Ⅰ, Xba Ⅰ and Xho Ⅰ, analysed by using agarose gel electrophoresis and detected by Gel-Pro Analyzer. Restriction enzyme maps revealed that 4 kinds of haplotypes exsited in L. erythopterus, 3 in L. russelli, 6 in L. argentimaculatus and 5 in L. johni. We also discussed the mechanism of this phenomenon based on restriction sites variant, calculated the nucleotide diversity of inter-species genetic distance. The polymorphic degrees of mtDNAs from above four snappers were 0. 002 9, 0. 005 5, 0. 001 2 and 0. 002 0 respectively.