文献类型：期刊文献

英文题名：Nanoplatinum-enclosed gold nanocores as catalytically promoted nanolabels for sensitive electrochemical immunoassay

作者：Zhou, Jun[2,3];Tang, Dianping[1,2];Hou, Li[2];Cui, Yuling[2];Chen, Huafeng[2];Chen, Guonan[2]

机构：[1]Fuzhou Univ, Dept Chem, Key Lab Anal & Detect Safety, Minist Educ, Fuzhou 350108, Peoples R China;[2]Fuzhou Univ, Dept Chem, Fujian Prov Key Lab Anal & Detect Food Safety, Fuzhou 350108, Peoples R China;[3]Guangdong Ocean Univ, Modern Biochem Ctr, Zhanjiang 524088, Peoples R China

年份：2012

卷号：751

起止页码：52

外文期刊名：ANALYTICA CHIMICA ACTA

收录：SCI-EXPANDED(收录号：WOS:000311013100004)、、EI(收录号：20124315608875)、Scopus(收录号：2-s2.0-84867741872)、WOS

基金：Support by the Research Fund for the National Science Foundation of Fujian Province (no. 2011J06003), the Doctoral Program of Higher Education of China (no. 20103514120003), the National Natural Science Foundation of China (nos. 21075019 and 41176079), the "973" National Basic Research Program of China (no. 2010CB732403), and the Program for Changjiang Scholars and Innovative Research Team in University (IRT1116) is gratefully acknowledged.

语种：英文

外文关键词：Electrochemical immunosensor; Nanoplatinum-enclosed gold nanocores; Nanolabels; Carcinoembryonic antigen; Carbon nanospheres-graphene hybrid nanostructures

外文摘要：Here we designed a new electrochemical immunoassay protocol for determination of carcinoembryonic antigen (CEA) using nanoplatinum-enclosed gold nanocores (Pt@Au) as catalytically promoted nanolabels on the carbon nanospheres and graphene-modified immunosensor. The Pt@Au nanolabels were synthesized and functionalized with monoclonal anti-CEA antibodies and glucose oxidase (GOx). Using the functional Pt@Au nanolabels as molecular tags, the assay was implemented relative to glucose-hydroquinone system with a sandwich-type immunoassay. Initially, the added glucose was oxidized to gluconolactone and H2O2 by the labeled GOx, and then the generated H2O2 was reduced with the help of platinum nanoparticles, leading to the production of oxygen. The self-produced oxygen could promote the re-oxidation of the glucose, thus resulting in the dual amplification of the electrochemical signal. Several nanolabels, such as multiarmed star-like platinum nanowires, hollow platinum nanospheres and Pt@Au nanostructures, were investigated for CEA detection and improved analytical features were obtained with the Pt@Au nanostructures. Under optimal conditions, the Pt@Au-based immunoassay displayed a wide working range from 0.001 to 120 ng mL(-1) with a low detection limit of 0.5 pg mL(-1) CEA at 3.s(B). Intra- and inter-assay coefficients of variation were <10.9%. The system was evaluated with 10 clinical serum samples, receiving good accordance with results from enzyme-linked immunosorbent assay method. (c) 2012 Elsevier B.V. All rights reserved.