文献类型：期刊文献

英文题名：Involvement and characterization of NLRCs and pyroptosis-related genes in Nile tilapia (Oreochromis niloticus) immune response

作者：Li, Qi[1,2];Jiang, Baijian[2];Zhang, Zhiqiang[2];Huang, Yongxiong[2];Xu, Zhou[2];Chen, Xinjin[2];Huang, Yu[2,3,4];Jian, Jichang[2,3,4];Yan, Qingpi[1]

机构：[1]Jimei Univ, Fisheries Coll, Xiamen, Peoples R China;[2]Guangdong Ocean Univ, Coll Fishery, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang, Peoples R China;[3]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;[4]Guangdong Prov Engn Res Ctr Aquat Anim Hlth Assess, Shenzhen, Peoples R China

年份：2022

卷号：130

起止页码：602

外文期刊名：FISH & SHELLFISH IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000867316400005)、、WOS

基金：Funding This work was supported by National Natural Science Foundation of China (No. 32073006, 32002426) , National Key R&D Program of China (No. 2018YFD0900501) , Fund of Southern Marine Science and Engi-neering Guangdong Laboratory (Zhanjiang) (No. ZJW-2019-06) .

语种：英文

外文关键词：Nile tilapia; NLRC; Pyroptosis; Streptococcus agalactiae; Aeromonas hydrophila; Lymphocyte

外文摘要：Pyroptosis is an inflammatory and programmed cell death initiated by the formation of the inflammasome, which consists of NLR, ASC, and Caspase. Pyroptosis has received growing attention due to its association with innate immunity and various diseases. However, the involvement and induction of the NLRCs and pyroptosis-related genes in fish immunity remain poorly studied. In this study, several NLRCs and pyroptosis-related genes in Nile tilapia (Oreochromis niloticus) were identified and characterized. Their involvement in bacterial infection and expression profiles in Nile tilapia lymphocyte responses were also assessed. Overall, three NLRC members (NOD1, NOD2, and NLRC3) and five pyroptosis-related genes (ASC1, Caspase1, Gsdme, NLRP3, and NLRP14) in Nile tilapia were cloned and characterized. The transcript levels of these molecules were broadly distributed in various tissues with comparatively high expression in the gills, intestine, and spleen. Their transcripts were also induced during Streptococcus agalactiae or Aeromonas hydrophila infection. Moreover, they were primarily expressed in T cells, NCCs, and Mo/M phi and showed antibacterial and partially antiviral responses. The present study lays a theoretical foundation for further investigation of the pyroptosis mechanisms in fish as well as the evolution of the antiviral roles of pyroptosis in vertebrates.