文献类型：期刊文献

英文题名：Iridoid Glycosides from Phlomis Medicinalis Diels: Optimized Extraction and Hemostasis Evaluation

作者：Lu, Sitong[1];Zhang, Zhaoyu[1];Liang, Youling[1];Zhong, Qingkun[1];Li, Guangfa[1];Hu, Zhang[1]

机构：[1]Guangdong Ocean Univ, Sch Chem & Environm Sci, Dept Appl Chem, Zhanjiang 524088, Guangdong, Peoples R China

年份：2022

卷号：19

期号：5

外文期刊名：CHEMISTRY & BIODIVERSITY

收录：SCI-EXPANDED(收录号：WOS:000789874300001)、、Scopus(收录号：2-s2.0-85129590623)、WOS

基金：The authors gratefully acknowledge the financial support by National Key Research and Development Program of China (2020YFD0901101), Project of Science and Technology Plan of Zhanjiang (2019A01017 and 2020A01026), Guangdong Ocean University Undergraduate Innovation Team Project (CXTD2021020).

语种：英文

外文关键词：Phlomis medicinalis; iridoid glycoside; ultrasound-assisted extraction; deep eutectic solvent; hemostasis activity

外文摘要：Phlomis medicinalis Diels, an important perennial herbal plant unique to the Qinghai-Tibet Plateau, is often used as Tibetan Materia Medicine Radix Phlomii for the treatment of cold, cough, and convergence trauma. In order to efficiently extract the iridoid glycosides from P. medicinalis, an ultrasound-assisted deep eutectic solvent extraction technique was employed. The main parameters influencing the extraction process were studied through single-factor tests and the extraction was optimized by using response surface methodology. The hemostasis activity of total iridoid glycosides (TIG) from P. medicinalis was evaluated in vitro and in mice. The optimization results revealed that the optimal process parameters were liquid-solid ratio 20 : 1, choline chloride-lactic acid concentration 79 %, and sonication time 34 min, under which a TIG extraction yield of 20.73 % was obtained. Meanwhile, high-performance liquid chromatography-photodiode array/mass spectrometry (HPLC-PDA/MS) was employed to characterize the optimized extract and indicated that TIG from P. medicinalis mainly consisted of sixteen reported iridoid glycosides with a total content of 91.22 %. The experimental results in vivo and in vitro indicated that TIG from P. medicinalis had strong hemostasis activities, which may be achieved by increasing the fibrinogen levels. Therefore, the ultrasound-assisted deep eutectic solvent extraction is an effective method to extract iridoid glycosides from P. medicinalis and they will be promising candidates to be developed for medical hemostasis agents.