文献类型：期刊文献

中文题名：激活和抑制GPR120基因对奶牛MAC-T细胞乳脂代谢及炎性反应的影响

英文题名：Effects of activating and suppressing GPR120gene on milk lipidmetabolism and inflammatory response of dairy cattle MAC-T cells

作者：宛麟[1];周佩瑶[1];牟泉宙[1];李鑫丽[1];王斯琦[1];李杨[1];何兴丽[1];王昭元[1];王梓[1];高梓强[1];赵志辉[2];沈冰蕾[1]

机构：[1]黑龙江八一农垦大学动物科技学院,黑龙江大庆163319;[2]广东海洋大学农学院,广东湛江524088

年份：2024

卷号：44

期号：12

起止页码：2655

中文期刊名：中国兽医学报

外文期刊名：Chinese Journal of Veterinary Science

收录：北大核心2023、、CSCD_E2023_2024、北大核心、CSCD

基金：黑龙江省自然科学基金面上资助项目(LH2022C066);黑龙江省博士后科研启动基金资助项目(LBH-Q21157)。

语种：中文

中文关键词：GPR120基因;MAC-T细胞;乳脂合成;炎性因子

外文关键词：GPR120gene;MAC-T cells;milk fat synthesis;inflammatory factors

中文摘要：GPR120作为受体蛋白可被长链脂肪酸(如ω-3脂肪酸,α-亚麻酸,二十碳五烯酸和二十二碳六烯酸)激活,在胃肠道肽的释放、炎症、脂肪生成、葡萄糖耐量,胰岛素敏感性等过程中发挥重要调控作用。为了研究GPR120基因对奶牛MAC-T细胞乳脂代谢及抗炎的协同调控作用,使用GPR120激活剂TUG-891与抑制剂AH7614对奶牛MACT细胞进行处理以及对LTA诱导的炎性奶牛MAC-T细胞进行处理检测乳脂合成关键基因及炎性因子的表达。结果显示,GPR120基因激活剂能显著提高乳脂合成关键基因胆固醇调节元件结合蛋白(SREBP1)和脂肪酸合成酶(FASN)的相对表达量,在炎性奶牛MAC-T细胞模型下能降低炎性因子白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的表达量,初步揭示GPR120协同调控奶牛乳脂与乳房炎症,为后续分子机制研究奠定了基础。

外文摘要：As a receptor protein,GPR120is activated by long-chain fatty acids such as omega-3fatty acids,alpha-linolenic acid,eicosapentaenoic acid,and docosahexaenoic acid.It plays an important regulatory role in gastrointestinal peptide release,inflammation,lipogenesis,glucose tolerance,and insulin sensitivity.In order to study the synergistic regulation of the GPR120gene on milk fat metabolism and its anti-inflammatory effects in dairy cow MAC-T cells,the GPR120activator TUG-891and the inhibitor AH7614were used to treat both dairy cow MAC-T cells and LTA-induced inflammatory dairy cow MAC-T cells.This treatment aimed to detect the expression of key genes involved in milk fat synthesis and inflammatory factors.The results showed that the GPR120gene activator significantly increased the relative expression levels of cholesterol regulatory element binding protein(SREBP1)and fatty acid synthase(FASN),key genes for milk fat synthesis.Additionally,the expression levels of the inflammatory factor interleukin-6(IL-6)and tumor necrosis factor-α(TNF-α)were reduced in the inflammatory dairy cow MAC-T cell model,which preliminatively reveals that GPR120co-regulates milk fat and mammary inflammation in dairy cows,thereby laying a foundation for subsequent molecular mechanism research.