文献类型：期刊文献

中文题名：不同方法纯化的尼罗罗非鱼下脚料蛋白酶解物锌螯合盐的理化特性

英文题名：Physicochemical properties of zinc chelating purified components by different method of tilapia scraps protein hydrolysate

作者：杨萍[1];李同刚[1];佘春霞[1];洪鹏志[1];周春霞[1]

机构：[1]广东海洋大学食品科技学院、广东普通高等学校水产品深加工重点实验室,广东湛江524088

年份：2015

卷号：39

期号：3

起止页码：447

中文期刊名：水产学报

外文期刊名：Journal of Fisheries of China

收录：CSTPCD、、北大核心2014、CSCD2015_2016、Scopus、北大核心、CSCD

基金：国家“八六三”高技术研究发展计划(2013AA102201);广东省高校重大科研项目培育计划(2014KZDXM039);省部产学研合作专项(2013B090800021)

语种：中文

中文关键词：尼罗罗非鱼下脚料;酶解物;锌螯合盐;纯化;抗氧化

外文关键词：tilapia scraps ; enzymatic hydrolysate ; zinc chelating salts; purification ; antioxidation

中文摘要：研究罗非鱼下脚料蛋白酶解物锌螯合盐(CH-0)及乙醇纯化(CH-Ⅰ)与透析纯化组分(CH-Ⅱ)的理化特性及体外自由基的清除能力,为多肽螯合盐的纯化研究提供理论基础。结果表明,CH-0的蛋白质含量(干基计)为71.86%,CH-Ⅰ和CH-Ⅱ分别为75.94%与78.83%;CH-0的锌含量为16.08%,CH-Ⅰ和CH-Ⅱ分别为14.49%与12.20%;凝胶层析结果显示,CH-Ⅰ、CH-Ⅱ与CH-0相比,有3个相同的主吸收峰,且大分子与小分子杂峰减少;氨基酸分析结果表明CH-Ⅱ中的Asp、Glu、Gly、Lys、Arg和His的含量均高于CH-Ⅰ,且都高于CH-0,与透析纯化相比,乙醇纯化必需氨基酸与支链氨基酸损失较大;CH-0、CH-Ⅰ和CH-Ⅱ对羟基自由基与DPPH自由基的清除率随浓度增强而增强,以IC50值计,CH-Ⅰ、CH-Ⅱ比CH-0的清除能力分别提高了8.92%、24.01%与38.06%、46.13%。罗非鱼下脚料,蛋白酶解物(EH10K)有较好的超氧阴离子自由基清除能力,浓度为10.00 mg/m L时清除率为69.27%,而CH-0、CH-Ⅰ与CH-Ⅱ则显示了负清除作用。纯化方法对罗非鱼下脚料蛋白酶解物锌螯合盐的理化特性有影响。

外文摘要：Tilapia scraps were hydrolyzed by neutral protease and flavourzyme, and the hydrolysate was fractionated through ultrafiltration membranes with a range of molecular weight cutoffs（MWCO） of 10 ku, to yield the fraction EH10K with MW distribution 〈 10 ku. The zinc chelating salts （CH-0） was produced by EH10K chelating with ZnSOa. CH- Ⅰ and CH-Ⅱ were the purified components from CH-0 by using 95% ethanol and dialysis （FW = 500 u） respectively. The formation of Zn^2＋ chelating salts of hydrolysate was confirmed by the UV-VIS spectra. The physicochemical properties of CH-0, CH- Ⅰ and CH-Ⅱ were analysed and their free radical scavenging activities were evaluated also. The results showed that CH-0 contained 71. 86% of protein （on dry basis） , 16. 08% of Zn, CH- Ⅰand CH-Ⅱ were 75. 94% and 14.49% ,78.83% and 12.20% respectively. The gel chromatography results showed that CH- Ⅰ and CH-Ⅱ had three same main absorption peaks compared to CH-0 but had little impurity peaks. The contents of Asp, Glu,Gly, Lys,Arg and His in CH- Ⅱ were high than those of CH- Ⅰ , and all were high than those of CH-0. The content of essential amino acid and branched chain amino acid in CH- Ⅱ were high than that in CH- Ⅰ. CH-0,CH- Ⅰ and CH-Ⅱ showed evident radical scavenging activity in a dose-dependent manner with the ICs0 values for hydroxyl radical and 1,1-diphenyl-2-pycrylhydrazyl （DPPH） radical being 9.08 and 6.07, 8.27 and 3.76,6.90 and 3.27 mg/mL respectively. The superoxide radical scavenging activity of EH10K was 69.27% at 10.00 mg/mL,CH-0,CH-Ⅰ and CH-Ⅱ showed no effect on superoxide radical scavenging but to oromote the oxidation