文献类型：期刊文献

中文题名：近海沉积物DlPCBs对斑马鱼胚胎EROD和cyp1a mRNA的影响

英文题名：EROD Activity and cyp1a mRNA Expression in Zebrafish Embryo Exposed to Marine Sediment DlPCBs Extract

作者：聂芳红[1];蔡吉榛[2];王小宁[2];林红英[2];Ravi Gooneratne[3];Anthony Hay[4];马驿[2];巨向红[2];郑锦庆[2];陈进军[2]

机构：[1]广东海洋大学食品科技学院,湛江524088;[2]广东海洋大学农学院,湛江524088;[3]林肯大学农业与生命学院,新西兰基督城7647;[4]康奈尔大学农业与生命科学学院,美国伊萨卡14853

年份：2016

卷号：11

期号：2

起止页码：364

中文期刊名：生态毒理学报

外文期刊名：Asian Journal of Ecotoxicology

收录：CSTPCD、、北大核心2014、CSCD2015_2016、北大核心、CSCD

基金：广东省科技计划国际合作项目(2010B050600004,2016A050502062)

语种：中文

中文关键词：类二噁英多氯联苯;斑马鱼胚胎;EROD酶;cyp1a;m;RNA;近海沉积物

外文关键词：dioxin-like polychlorinated biphenyls; zebrafish embryo; EROD; cyp1a mRNA; marine sediment

中文摘要：为探究湛江近海域海洋沉积物中类二噁英多氯联苯（dioxin-like polychlorinated biphenyls,Dl PCBs）的生物学毒性效应,选取湛江近海域2个地点（近工业区TS和近生活区JSW）采集沉积物样品,制备近海域沉积物Dl PCBs提取物,将斑马鱼胚胎暴露于不同浓度的Dl PCBs提取物,测定斑马鱼胚胎7-乙氧基异吩恶唑脱乙基酶（ethoxyresorufin-O-deethylase,EROD）活性和cyp1a m RNA相对表达量。结果发现,JSW采样点Dl PCBs提取物染毒组EROD酶活性变化与TS采样点Dl PCBs提取物一致,在各染毒浓度下,斑马鱼仔鱼EROD酶活性为对照组的1.1～1.8倍。TS和JSW采样点不同浓度Dl PCBs提取物暴露斑马鱼胚胎96 h后,使斑马鱼仔鱼cyp1a m RNA相对表达量是对照组的3.36～19.45倍。说明一定浓度的近海沉积物Dl PCBs能诱导斑马鱼仔鱼EROD酶活性和cyp1a m RNA表达量升高,且呈现浓度-效应关系。

外文摘要：To study the bio-toxicity of dioxin-like polychlorinated biphenyls（Dl PCBs） in marine sediment, the sediments from Zhanjiang industrial and living area（TS and JSW） were collected and extracted focusing on Dl PCBs, re-spectively. Then a range of zebrafish embryos were exposed to different concentrations of the sediment Dl PCBs extracts to examine the ethoxyresorufin-O-deethylase（EROD） activity and the expression of cyp1 a m RNA. The results showed that the Dl PCBs extract from TS and JSW sediments had similarity to cause marked induction of EROD in the zebrafish embryos.The EROD activities in zebrafish embryos induced by different concentrations of the Dl PCBs extract were 1.1 to 1.8 times higher than that in the control embryos, respectively, and after the zebrafish embryos were exposed to different concentrations of the Dl PCBs for 96 h, the juvenile cyp1 a m RNA relative expressions were significantly enhanced by 3.36 to 19.45 times of that in the control group, respectively. It is indicated that certain concentrations of the Dl PCBs extract increased EROD activity and cyp1 a m RNA in zebrafish larvae, with a concentration-effect relationship.