文献类型：期刊文献

英文题名：Antioxidant activity of bigeye tuna (Thunnus obesus) head protein hydrolysate prepared with Alcalase

作者：Yang, Ping[1];Ke, Hongqiao[1];Hong, Pengzhi[1];Zeng, Shaokui[1];Cao, Wenhong[1]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524025, Peoples R China

年份：2011

卷号：46

期号：12

起止页码：2460

外文期刊名：INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000297257300002)、、EI(收录号：20114714537725)、Scopus(收录号：2-s2.0-81255147401)、WOS

基金：Financial supports for this research were Guangdong Natural Science Foundation of China with grant no. 10152408801000031.

语种：英文

外文关键词：Free radical scavenging activity; hydrolysate; lipid peroxidation; molecular weight distribution; tuna head

外文摘要：The antioxidant activities of tuna head protein hydrolysate (THPH) prepared with Alcalase were evaluated. THPH showed evident radical scavenging activity in a dose-dependent manner with the IC50 values for 1,1-diphenyl-2-pycrylhydrazyl (DPPH), superoxide and hydroxyl radicals being 1.34, 1.20 and 2.84 mg mL-1, respectively, and its reducing power was 0.948 at 12.5 mg mL-1. THPH showed good inhibitory activity in soybean oil peroxidation after accelerated oxidation at 60 degrees C, and the oils with 0.01%, 0.05% and 0.1% THPH had significantly (P < 0.05) lower peroxide values than the control, after storage at 60 degrees C. Moreover, the inhibited oxidation effect of 0.1% THPH was similar to that of 0.01% butylated hydroxytoluene (BHT). The molecular weight distribution of THPH revealed that 70.5% of the total amount was peptides with molecular weight lower than 5000 Da, composing mostly of low molecular weight peptides located at 10202585 Da (30.78%) and 2411020 Da (37.15%).