文献类型：期刊文献

英文题名：Transcriptomic and metabolomic analyses reveal sex-related differences in the gonads of Pinctada fucata martensii

作者：Fang, Jiaying[1];Yang, Chuangye[1,2,3,4];Liao, Yongshan[5];Wang, Qingheng[1];Deng, Yuewen[1,2,3,4,5]

机构：[1]Guangdong Ocean Univ, Fisheries Coll, Zhanjiang 524088, Peoples R China;[2]Guangdong Sci & Innovat Ctr Pearl Culture, Zhanjiang 524088, Peoples R China;[3]Pearl Breeding & Proc Engn Technol Res Ctr Guangdo, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Zhanjiang 524088, Peoples R China;[5]Guangdong Ocean Univ, Pearl Res Inst, Zhanjiang, Peoples R China

年份：2024

卷号：52

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY D-GENOMICS & PROTEOMICS

收录：SCI-EXPANDED(收录号：WOS:001321071800001)、、Scopus(收录号：2-s2.0-85200773124)、WOS

基金：This work was supported by National Key Research and Development Program of China (Grant No. 2022YFD2401203), the program for scientific research start-up funds of Guangdong Ocean University (Grant No. 060302022304), Department of Education of Guangdong Province (Grant No. 2021KCXTD026), the earmarked fund for CARS-49, and Technology Program of Guangdong Zhanjiang (Grant No. 2022A01010). We are very grateful to Marine Pearl Science and Technology Backyard in Leizhou of Guangdong for collecting samples. The authors would like to thank TopEdit (www.topeditsci.com) for its linguistic assistance during the preparation of this manuscript. Metabolomic analysis was assisted by Biotree Biotech Co., Ltd. (Shanghai, China).

语种：英文

外文关键词：Pinctada fucata martensii; Gender; Transcriptome; Metabolome

外文摘要：Pinctada fucata martensii is an economically important bivalve mollusk, as this species makes a major contribution to seawater pearl production. Pearl production efficiency varies between the sexes of P. f. martensii, but many aspects of the molecular mechanisms underlying sex determination and sex differentiation in P. f. martensii remain unclear. Here, transcriptomic and metabonomic analyses were conducted to identify the major genes and metabolic changes associated with sex determination and gametogenesis. We identified a total of 3426 differentially expressed genes (DEGs) between females and males. These included Fem-1c and Foxl2, which are involved in sex determination and sex differentiation, and SOHLH2, Nanos1 and TSSK4, which are involved in gametogenesis. We also identified a total of 5231 significant differential metabolites (SDMs) between females and males. These DEGs were enriched in 47 metabolic pathways, including "ABC transporters," "purine metabolism," and "glycerophospholipid metabolism." Our findings provide new insights into the molecular mechanisms underlying sex determination, sex differentiation, and gametogenesis and will aid future studies of P. f. martensii.