文献类型：期刊文献

英文题名：Molecular characterization of CHST11 and its potential role in nacre formation in pearl oyster Pinctada fucata martensii

作者：Wang, Qingheng[1];Yang, Chuangye[1];Hao, Ruijuan[1];Zheng, Zhe[1];Jiao, Yu[1];Du, Xiaodong[1];Deng, Yuewen[1];Huang, Ronglian[1]

机构：[1]Guangdong Ocean Univ, Fishery Coll, Zhanjiang 524088, Guangdong, Peoples R China

年份：2017

卷号：28

起止页码：113

外文期刊名：ELECTRONIC JOURNAL OF BIOTECHNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000405483200016)、、EI(收录号：20172803923124)、Scopus(收录号：2-s2.0-85021811852)、WOS

基金：The study was financially supported by grants from the National Natural Science Foundation of China (31372526, 31272635 and 41206141).

语种：英文

外文关键词：Biomineral; Biomineralization; Carbohydrate sulfotransferase 11; Chondroitin sulfate; Chondroitin-4-sulfotransferase-1 (C4ST-1); Mantle tissue; Molluscs; Proteoglycans; Real-time PCR; RNA interference

外文摘要：Background: C4ST-1 catalyzes the transfer of sulfate groups in the sulfonation of chondroitin during chondroitin sulfate synthesis. Chondroitin sulfate consists of numerous copies of negatively charged sulfonic acid groups that participate in the nucleation process of biomineralization. In the present study, we obtained two CHST11 genes (PmCHST11a and PmCHST11b) which encoded the C4ST-1 and explored the functions of these genes in the synthesis of chondroitin sulfate and in the formation of the nacreous layer of shells. Results: Both PmCHST11a and PmCHST11b had a sulfotransferase-2 domain, a signal peptide and a transmembrane domain. These properties indicated that these genes localize in the Golgi apparatus. Real-time PCR revealed that both PmCHST11a and PmCHST11b were highly expressed in the central zone of the mantle tissue. Inhibiting PmCHST11a and PmCHST11b via RNA interference significantly decreased the expression levels of these genes in the central zone of the mantle tissue and the concentration of chondroitin sulfate in extrapallial fluid. Moreover, shell nacre crystallized irregularly with a rough surface after RNA interference. Conclusions: This study indicated that PmCHST11a and PmCHST11b are involved in the nacre formation of Pinctada fucata martensii through participating in the synthesis of chondroitin sulfate. (C) 2017 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.