文献类型：期刊文献

英文题名：Simultaneous Quantification of Aflatoxin B-1, T-2 Toxin, Ochratoxin A and Deoxynivalenol in Dried Seafood Products by LC-MS/MS

作者：Deng, Yijia[1];Wang, Yaling[1];Deng, Qi[1];Sun, Lijun[1];Wang, Rundong[2];Wang, Xiaobo[1];Liao, Jianmeng[3];Gooneratne, Ravi[4]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol,Guangdong Prov Engn Techn, Guangdong Prov Engn Technol Res Ctr Marine Food,G, Key Lab Adv Proc Aquat Prod Guangdong Higher Educ, Zhanjiang 524088, Peoples R China;[2]Lingnan Normal Univ, Sch Chem & Chem Engn, Zhanjiang 524048, Peoples R China;[3]Zhanjiang Inst Food & Drug Control, Zhanjiang 524022, Peoples R China;[4]Lincoln Univ, Fac Agr & Life Sci, Dept Wine Food & Mol Biosci, POB 85084, Lincoln 7647, New Zealand

年份：2020

卷号：12

期号：8

外文期刊名：TOXINS

收录：SCI-EXPANDED(收录号：WOS:000564158700001)、、WOS

基金：This research was funded by National Natural Science Foundation of China [Grant no. 31871898] and the Guangdong Provincial Special Fund for Modern Agricultural Industry Technology Innovation Teams [2019KJ149, 2019KJ151]. The APC was funded by the Guangdong Ocean University program for scientific research start-ups [R19050].

语种：英文

外文关键词：mycotoxins; dried seafood; liquid chromatography-tandem mass spectrometry; method validation; ultrasound-assisted extraction

外文摘要：Mycotoxins are secondary metabolites produced by fungi. These contaminate dried seafoods during processing and storage and represent a potential health hazard for consumers. A sensitive, selective and accurate liquid chromatography/tandem mass spectrometry (LC-MS/MS) method was established for simultaneous quantification of four common mycotoxins (aflatoxin B-1(AFB(1)), T-2 toxin (T-2), ochratoxin A (OTA) and deoxynivalenol (DON)) in dried shrimp, dried fish and dried mussel products. Mycotoxins were extracted from dried seafood samples by acetonitrile/water (85/15,v/v), subjected to ultrasound for 60 min at 20 degrees C and cleaned up by defatting with n-hexane. The sample matrix affected the linearity of detection (R-2 >= 0.9974). The limit of detection (LOD) and limit of quantification (LOQ) in dried seafood products varied from 0.1 to 2.0 mu g center dot kg(-1)and 0.3 to 5.0 mu g center dot kg(-1), respectively. The method was validated by spiking samples with specific mycotoxin levels, and the recoveries, intra-relative standard deviation (RSDs) and inter-RSDs ranged between 72.2-98.4%, 2.8-10.6%, and 5.5-15.4%, respectively. This method was used to analyze 40 dried seafood products purchased from the Zhanjiang seafood market. Results of this product sampling showed that while no DON was detected, AFB(1), T-2 and OTA were detected in 30.8%, 17.5% and 33.3% of the samples, respectively. AFB(1), T-2 and OTA concentrations varied at 0.58-0.89, 0.55-1.34 and 0.36-1.51 mu g center dot kg(-1), respectively. Relatively high frequency of contamination and the presence of AFB(1), OTA and T-2 residues indicate the need to monitor mycotoxins in dried seafood products.