文献类型：期刊文献

英文题名：Novel Antioxidant Peptides from Crassostrea Hongkongensis Improve Photo-Oxidation in UV-Induced HaCaT Cells

作者：Zhang, Chen[1];Lv, Jiatong[1];Qin, Xiaoming[1,2,3,4,5,6];Peng, Zhilan[1];Lin, Haisheng[1,2,3,4,5,6]

机构：[1]Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China;[2]Guangdong Prov Key Lab Aquat Product Proc & Safet, Zhanjiang 524088, Peoples R China;[3]Natl Res & Dev Branch Ctr Shellfish Proc, Zhanjiang 524088, Peoples R China;[4]Guangdong Prov Engn Lab Marine Biol Prod, Zhanjiang 524088, Peoples R China;[5]Guangdong Prov Engn Technol Res Ctr Marine Food, Zhanjiang 524088, Peoples R China;[6]Dalian Polytech Univ, Collaborat Innovat Ctr Seafood Deep Proc, Dalian 116034, Peoples R China

年份：2022

卷号：20

期号：2

外文期刊名：MARINE DRUGS

收录：SCI-EXPANDED(收录号：WOS:000765098100001)、、Scopus(收录号：2-s2.0-85123881883)、WOS

基金：This research was financially supported by the China Agricultural Research System of MOF and MARA, High-Value Aquatic Products Processing and Utilization Innovation Team of Higher Education Institutions of Guangdong Province (GDOU2016030503).

语种：英文

外文关键词：oyster; peptides; photo-oxidation; HaCaT cells

外文摘要：Enzymatic hydrolysates from Oysters (OAH) display multiple biological activities. Previously, a 3~5 KDa oyster ultrafiltration component (OUP) showed a high property of preventing skin oxidation. Subsequently, we identified specific peptides with such activity. OUP was fractionated stepwise by Sephadex-G25 and RP-HPLC, and active fractions were screened using UV-irradiated HaCaT cells. The most active fractions (OP5-3) were analyzed by LC-MS/MS and a total of 17 peptides were identified. Results from mass spectrometry showed that OP5-3 consisted of peptides with a molecular weight range of 841.51-1786.92 Da. Six of these peptides were synthesized for validating the activity of resisting skin oxidation in the same cell model. All six peptides showed varying degrees of antioxidant activity, while pretreatment of HaCaT cells with AIVAEVNEAAK alleviated UV cytotoxicity, inhibited metalloproteinase 1 (MMP-1) expression, and showed the highest activity to resist UV-induced skin photo-oxidation among these peptides. In addition, results from molecular docking analysis of MMP-1 with AIVAEVNEAAK showed that AIVAEVNEAAK suppresses its enzymatic activity by directly interacting with MMP-1 and thus exhibit anti-photoaging activity.