文献类型：期刊文献

英文题名：Transcriptional alterations induced by binary mixtures of ethinylestradiol and norgestrel during the early development of zebrafish (Danio rerio)

作者：Liang, Yan-Qiu[1,2];Huang, Guo-Yong[1];Zhao, Jian-Liang[1];Shi, Wen-Jun[1];Hu, Li-Xin[1];Tian, Fei[1];Liu, Shuang-Shuang[1];Jiang, Yu-Xia[1];Ying, Guang-Guo[1,3]

机构：[1]Chinese Acad Sci, Guangzhou Inst Geochem, CAS Res Ctr PRD Environm Pollut & Control, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China;[2]Guangdong Ocean Univ, Coll Chem & Environm, Zhanjiang 524088, Peoples R China;[3]South China Normal Univ, MOE Key Lab Environm Theoret Chem, Environm Res Inst, Guangzhou 510006, Guangdong, Peoples R China

年份：2017

卷号：195

起止页码：60

外文期刊名：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY

收录：SCI-EXPANDED(收录号：WOS:000398875100007)、、WOS

基金：The authors would like to acknowledge the financial support from the National Natural Science Foundation of China (41273119, U1133005, 41201522, and U1401235). This is a Contribution No. IS 2349 from GIG CAS.

语种：英文

外文关键词：Gene transcription; Mixture effects; Ethinylestradiol; Norgestrel

外文摘要：Synthetic estrogens and progestins are commonly used in human and veterinary medicine. After use, they reach aquatic environments via discharge of wastewaters from human and animals, thus posing potential risks to organisms. So far, very little is known about their combined effects in aquatic organisins. The aim of this study was to investigate the effects of binary mixtures of ethinylestradiol (EE2) and norgestrel (NGT) on embryonic zebrafish (Danio rerio) by measuring transcriptional alterations. Zebrafish embryos were exposed to EE2 and NGT alone or in combination at concentrations between 36 and 5513 ng L-1 for 96 h post-fertilization (hpf). The results showed that most of gene transcriptions of hypothalamic-pituitary-gonadal axis (e.g., Pgr, Mpr alpha, Esr1, Esr2a, Vtg1, Ar, Cyp11b, Star, Gnrh3 and Fshb) and circadian rhythm signaling (e.g., Cry1a, Cry2a, Cry2b, Per3, Arnt1lb, Arntl2, Clock1a, Cry3 and Cry4) displayed most pronounced alterations in the mixtures as compared to single EE2 and NGT exposures. This finding suggests exposure to the binary mixtures of EE2 and NGT produced significantly enhanced effects in fish as compared to single chemical exposures, and their coexistence could have significant environmental implications. (C) 2017 Elsevier Inc. All rights reserved.