文献类型：期刊文献

英文题名：Molecular characterization of LvAV in response to white spot syndrome virus infection in the Pacific white shrimp (Litopenaeus vannamei)

作者：He, Shulin[1];Song, Lei[2];Qian, Zhaoying[3];Hou, Fujun[1];Liu, Yongjie[1];Wang, Xianzong[1];Peng, Zhangming[4];Sun, Chengbo[4];Liu, Xiaolin[1]

机构：[1]Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China;[2]Mil Econ Acad Airforce, Dept Polit Teaching Off, Wuhan 430035, Peoples R China;[3]Guizhou Univ Finance & Econ, Sch Resource & Environm Management, Guiyang 550025, Guizhou, Peoples R China;[4]Guangdong Ocean Univ, Coll Fisheries, Zhanjiang 524088, Guangdong, Peoples R China

年份：2015

卷号：51

期号：1

起止页码：48

外文期刊名：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY

收录：SCI-EXPANDED(收录号：WOS:000357906800006)、、WOS

基金：Comments from Dino Mcmahon and anonymous reviewers have greatly improved the manuscript. This work was supported by the Agricultural Science and Technology Achievement Transformation Fund Project of Ministry of Science and Technology, China (no. 2012GB2E200361), the National Fundamental Research Program, China (no. 2006AA10A406), the Key Laboratory of Marine Biology, Institute of Oceanology, Chinese Academy of Sciences and the Northwest A&F University experimental demonstration station (base) and innovation of science and technology achievement transformation project (no. XNY2013-4).

语种：英文

外文关键词：Litopenaeus vannamei; LvAV; C-type lectin; WSSV; Vibrio parahaemolyticus

外文摘要：Litopenaeus vannamei is the most important farmed shrimp species globally, but its production is affected by several factors, including infectious disease. White spot syndrome virus (WSSV), in particular, causes significant shrimp losses. To understand the shrimp's immune response against WSSV, we cloned LvAV from L. vannamei and analyzed its expression pattern in different tissues, in addition to its expression following infection. We employed dsRNA and recombinant (r)LvAV to explore the potential role of LvAV in shrimp immunity when infected with WSSV. We find that LvAV is a C-type Lectin composed of 176 amino acids with a signal peptide and a specific C-type Lectin-type domain (CTLD). It shares 81% amino acid similarity with PmAV, an antiviral-like C-type Lectin from Penaeus monodom, and it is highly expressed in the hepatopancreas. Its expression is affected by infection with both WSSV and V. parahaemolyticus. Significantly, injection with rLvAV slowed WSSV replication, while injection with LvAV dsRNA initially led to enhanced virus propagation. Surprisingly, LvAV dsRNA subsequently led to a dramatic decrease in viral load in the later stages of infection, suggesting that LvAV may be subverted by WSSV to enhance viral replication or immune avoidance. Our results indicate that LvAV plays an important, but potentially complex role in the Pacific white shrimp's immune defense. (C) 2015 Elsevier Ltd. All rights reserved.